Human BCL2 Associated X Protein ELISA Kit | BAX elisa kit

Human BCL2 Associated X Protein ELISA Kit

Cat. Num. AAA728263

• Reactivity: Human
• Synonyms: BCL2 Associated X Protein; Human BCL2 Associated X Protein ELISA Kit; BAX elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive or Sandwich
• Detection Range: 50-1000pg/mL
• Sensitivity: 1.0pg/mL
• Highest Standards: 1000pg/mL
• Concentration of Standards: Concentration of Standards
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Product Categories/Family for BAX elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 975870
• NCBI GeneID: 24887
• Molecular Weight: – Da
• NCBI Official Full Name: bax, partial
• NCBI Official Synonym Full Names: Bcl2-associated X protein
• NCBI Official Symbol: Bax
• NCBI Protein Information: apoptosis regulator BAX
• UniProt Protein Name: Apoptosis regulator BAX
• Protein Family: Protein
• UniProt Gene Name: Bax
• UniProt Entry Name: BAX_RAT

NCBI Description

Bcl2-related gene; involved in the regulation of apoptotic cell death [RGD, Feb 2006]

Uniprot Description

BAX: Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. Homodimer. Forms higher oligomers under stress conditions. Interacts with BCL2L11. Interaction with BCL2L11 promotes BAX oligomerization and association with mitochondrial membranes, with subsequent release of cytochrome c. Forms heterodimers with BCL2, E1B 19K protein, BCL2L1 isoform Bcl-X(L), BCL2L2, MCL1 and A1. Interacts with SH3GLB1 and HN. Interacts with SFN and YWHAZ; the interaction occurs in the cytoplasm. Under stress conditions, JNK-mediated phosphorylation of SFN and YWHAZ, releases BAX to mitochondria. Isoform Sigma interacts with BCL2A1 and BCL2L1 isoform Bcl-X(L). Interacts with RNF144B, which regulates the ubiquitin-dependent stability of BAX. Interacts with CLU under stress conditions that cause a conformation change leading to BAX oligomerization and association with mitochondria. Does not interact with CLU in unstressed cells. Interacts with FAIM2/LFG2. Interacts with human cytomegalovirus/HHV-5 protein vMIA/UL37. Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro- myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T- cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines. Belongs to the Bcl-2 family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Tumor suppressor; Apoptosis; Membrane protein, integral

Cellular Component: mitochondrial permeability transition pore complex; endoplasmic reticulum membrane; mitochondrion; endoplasmic reticulum; cell; nuclear envelope; cytosol; pore complex; mitochondrial outer membrane; membrane; cytoplasm; mitochondrial membrane; intracellular; nucleus

Molecular Function: BH domain binding; identical protein binding; protein binding; protein homodimerization activity; channel activity; protein heterodimerization activity; heat shock protein binding; chaperone binding; protein complex binding; BH3 domain binding; lipid binding

Biological Process: hypothalamus development; positive regulation of apoptosis; regulation of cell cycle; response to toxin; myeloid cell homeostasis; germ cell programmed cell death; homeostasis of number of cells; B cell apoptosis; post-embryonic development; germ cell development; regulation of mammary gland epithelial cell proliferation; spermatid differentiation; development of secondary sexual characteristics; regulation of mitochondrial membrane potential; protein insertion into mitochondrial membrane during induction of apoptosis; regulation of neuron apoptosis; establishment and/or maintenance of transmembrane electrochemical gradient; negative regulation of neuron apoptosis; kidney development; negative regulation of protein binding; response to corticosterone stimulus; response to drug; inner mitochondrial membrane organization and biogenesis; nervous system development; release of cytochrome c from mitochondria; outer mitochondrial membrane organization and biogenesis; positive regulation of B cell apoptosis; regulation of protein homodimerization activity; cellular respiration; vagina development; protein oligomerization; fertilization; DNA damage response, signal transduction resulting in induction of apoptosis; induction of apoptosis via death domain receptors; retina development in camera-type eye; negative regulation of fibroblast proliferation; reduction of endoplasmic reticulum calcium ion concentration; glycosphingolipid metabolic process; cerebral cortex development; response to ionizing radiation; mitochondrial fragmentation during apoptosis; regulation of nitrogen utilization; post-embryonic camera-type eye morphogenesis; regulation of protein heterodimerization activity; positive regulation of pigmentation; apoptosis; T cell homeostatic proliferation; negative regulation of peptidyl-serine phosphorylation; positive regulation of apoptosis involved in mammary gland involution; neuron migration; regulation of caspase activity; response to salt stress; release of matrix enzymes from mitochondria; negative regulation of cell proliferation; positive regulation of protein oligomerization; apoptotic mitochondrial changes; B cell homeostatic proliferation; ovarian follicle development; B cell homeostasis; positive regulation of neuron apoptosis; response to wounding; response to gamma radiation; response to axon injury; B cell negative selection; protein homooligomerization; leukocyte homeostasis; caspase activation; transformed cell apoptosis; mitochondrial fusion; male gonad development; Sertoli cell proliferation; response to cocaine; limb morphogenesis; odontogenesis of dentine-containing teeth; cell proliferation; neuron apoptosis; response to copper ion; homeostasis of number of cells within a tissue; spermatogenesis; blood vessel remodeling; retinal cell programmed cell death; brain development; positive regulation of release of sequestered calcium ion into cytosol; caspase activation via cytochrome c; response to DNA damage stimulus; sex differentiation