Typical Testing Data/Standard Curve (for reference only)
Rabbit BCL2 Associated X Protein ELISA Kit | BAX elisa kit
Rabbit BCL2 Associated X Protein ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
INTENDED USE This BAX ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit BAX. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY BAX ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BAX antibody and an BAX-HRP conjugate. The assay sample and buffer are incubated together with BAX-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BAX concentration since BAX from samples and BAX-HRP conjugate compete for the anti-BAX antibody binding site. Since the number of sites is limited, as more sites are occupied by BAX from the sample, fewer sites are left to bind BAX-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BAX concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Bcl2-related gene; involved in the regulation of apoptotic cell death [RGD, Feb 2006]
Uniprot Description
BAX: Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. Homodimer. Forms higher oligomers under stress conditions. Interacts with BCL2L11. Interaction with BCL2L11 promotes BAX oligomerization and association with mitochondrial membranes, with subsequent release of cytochrome c. Forms heterodimers with BCL2, E1B 19K protein, BCL2L1 isoform Bcl-X(L), BCL2L2, MCL1 and A1. Interacts with SH3GLB1 and HN. Interacts with SFN and YWHAZ; the interaction occurs in the cytoplasm. Under stress conditions, JNK-mediated phosphorylation of SFN and YWHAZ, releases BAX to mitochondria. Isoform Sigma interacts with BCL2A1 and BCL2L1 isoform Bcl-X(L). Interacts with RNF144B, which regulates the ubiquitin-dependent stability of BAX. Interacts with CLU under stress conditions that cause a conformation change leading to BAX oligomerization and association with mitochondria. Does not interact with CLU in unstressed cells. Interacts with FAIM2/LFG2. Interacts with human cytomegalovirus/HHV-5 protein vMIA/UL37. Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro- myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T- cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines. Belongs to the Bcl-2 family. 8 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Tumor suppressor; Apoptosis; Mitochondrial
Cellular Component: mitochondrial permeability transition pore complex; endoplasmic reticulum membrane; mitochondrion; cell; endoplasmic reticulum; nuclear envelope; cytosol; pore complex; mitochondrial outer membrane; membrane; mitochondrial membrane; cytoplasm; intracellular; nucleus
Molecular Function: BH domain binding; identical protein binding; protein binding; protein homodimerization activity; channel activity; protein heterodimerization activity; heat shock protein binding; chaperone binding; protein complex binding; BH3 domain binding; lipid binding
Biological Process: hypothalamus development; regulation of cell cycle; positive regulation of apoptosis; response to toxin; myeloid cell homeostasis; germ cell programmed cell death; homeostasis of number of cells; B cell apoptosis; post-embryonic development; germ cell development; regulation of mammary gland epithelial cell proliferation; spermatid differentiation; regulation of mitochondrial membrane potential; development of secondary sexual characteristics; protein insertion into mitochondrial membrane during induction of apoptosis; regulation of neuron apoptosis; establishment and/or maintenance of transmembrane electrochemical gradient; negative regulation of neuron apoptosis; kidney development; negative regulation of protein binding; response to corticosterone stimulus; inner mitochondrial membrane organization and biogenesis; response to drug; nervous system development; release of cytochrome c from mitochondria; positive regulation of B cell apoptosis; outer mitochondrial membrane organization and biogenesis; regulation of protein homodimerization activity; cellular respiration; vagina development; protein oligomerization; fertilization; DNA damage response, signal transduction resulting in induction of apoptosis; induction of apoptosis via death domain receptors; retina development in camera-type eye; negative regulation of fibroblast proliferation; reduction of endoplasmic reticulum calcium ion concentration; glycosphingolipid metabolic process; response to ionizing radiation; mitochondrial fragmentation during apoptosis; cerebral cortex development; regulation of nitrogen utilization; post-embryonic camera-type eye morphogenesis; positive regulation of pigmentation; regulation of protein heterodimerization activity; apoptosis; T cell homeostatic proliferation; positive regulation of apoptosis involved in mammary gland involution; neuron migration; negative regulation of peptidyl-serine phosphorylation; regulation of caspase activity; release of matrix enzymes from mitochondria; response to salt stress; negative regulation of cell proliferation; positive regulation of protein oligomerization; apoptotic mitochondrial changes; B cell homeostatic proliferation; ovarian follicle development; B cell homeostasis; positive regulation of neuron apoptosis; response to wounding; response to gamma radiation; B cell negative selection; response to axon injury; protein homooligomerization; leukocyte homeostasis; caspase activation; transformed cell apoptosis; mitochondrial fusion; male gonad development; Sertoli cell proliferation; response to cocaine; limb morphogenesis; odontogenesis of dentine-containing teeth; cell proliferation; neuron apoptosis; response to copper ion; homeostasis of number of cells within a tissue; spermatogenesis; blood vessel remodeling; retinal cell programmed cell death; positive regulation of release of sequestered calcium ion into cytosol; brain development; caspase activation via cytochrome c; response to DNA damage stimulus; sex differentiation
Research Articles on BAX
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Product Notes
The Rabbit BAX bax (Catalog #AAA732916) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA732916 ELISA Kit recognizes Rabbit BAX. It is sometimes possible for the material contained within the vial of "BCL2 Associated X Protein, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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