Typical Testing Data/Standard Curve (for reference only)
Mouse Transcription Factor A, Mitochondrial ELISA Kit | TFAM elisa kit
Mouse TFAM (Transcription Factor A, Mitochondrial) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TFAM. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TFAM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TFAM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse TFAM. You can calculate the concentration of Mouse TFAM in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a key mitochondrial transcription factor containing two high mobility group motifs. The encoded protein also functions in mitochondrial DNA replication and repair. Sequence polymorphisms in this gene are associated with Alzheimer's and Parkinson's diseases. There are pseudogenes for this gene on chromosomes 6, 7, and 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
Uniprot Description
TFAM: regulates mitochondrial transcription. Induced in humans by caloric restriction. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Activates transcription by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Involved in cisplatin resistance. Interacts with TFB1M and TFB2M.
Protein type: DNA-binding; Transcription regulation
Chromosomal Location of Human Ortholog: 10q21
Cellular Component: cytosol; mitochondrial matrix; mitochondrion; nucleus
Molecular Function: chromatin binding; DNA bending activity; heat shock protein binding; protein binding; transcription factor activity
Biological Process: DNA-dependent DNA replication; gene expression; mitochondrial respiratory chain complex assembly; mitochondrion organization and biogenesis; organelle organization and biogenesis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of transcription from RNA polymerase I promoter; transcription from mitochondrial promoter; transcription from RNA polymerase II promoter; transcription initiation from mitochondrial promoter
Research Articles on TFAM
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Product Notes
The Mouse TFAM tfam (Catalog #AAA2533527) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2533527 ELISA Kit recognizes Mouse TFAM. It is sometimes possible for the material contained within the vial of "Transcription Factor A, Mitochondrial, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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