BAMBI recombinant protein

BAMBI Protein, Mouse, Recombinant (hFc Tag)

Cat. Num. AAA8123018

• Gene Names: Bambi; 2610003H06Rik
• Purity: >90% as determined by SDS-PAGE
• Synonyms: BAMBI; BAMBI Protein; Mouse; Recombinant (hFc Tag); Mouse BAMBI/NMA Protein (Fc Tag); 2610003H06Rik Protein; BMP and activin membrane-bound inhibitor; BAMBI recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ala152

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 27
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the mouse BAMBI (Q9D0L6)(Met1-Ala152) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for BAMBI recombinant protein

Background: BMP and activin membrane-bound inhibitor (BAMBI) is a transmembrane glycoprotein that is a pseudoreceptor of type 1 receptors. BAMBI structurally lacks intracellular serine/ threonine kinase domain but with an extracellular domain and a short cytoplasmic region that share sequence similarities with type 1 receptors, whose members have functions in signal transduction in various developing and pathological processes. BAMBI competes with the type 1 receptor, a receptor of the transforming growth factor-beta (TGF-beta), through functioning as negative regulators of TGF-beta by limiting the signaling range of the TGF-beta family during early embryogenesis. The expression of BAMBI can be induced by accumulated beta-catenin and BMP. The expression level of BAMBI was found aberrantly elevated in most colorectal and hepatocellular carcinomas relative to the corresponding non-cancerous tissues. It suggestes that beta-catenin and TGF-beta interfere growth arrest by inducing the expression of BAMBI, and this may contribute to colorectal and hepatocellular tumorigenesis.

References

Sekiya T, et al. (2003) Identification of BMP and Activin Membrane-bound Inhibitor (BAMBI), an Inhibitor of Transforming Growth Factor- Signaling, as a Target of the -Catenin Pathway in Colorectal Tumor Cells. The Journal of Biological Chemistry. 279:6840-6. Shi YG, et al. (2003) Mechanisms of TGF- Signaling from Cell Membrane to the Nucleus. Cell. 113(6): 685-700.Wanninger J, et al. (2011) Adiponectin induces the transforming growth factor decoy receptor BAMBI in human hepatocytes. FEBS Lett. 585(9):1338-44.

NCBI and Uniprot Product Information

• NCBI GI #: 33468995
• NCBI GeneID: 68010
• NCBI Accession #: NP_080781.1
• NCBI GenBank Nucleotide #: NM_026505.2
• UniProt Accession #: Q9D0L6; Q3TP85; Q91XS6
• Molecular Weight: 29,198 Da
• NCBI Official Full Name: BMP and activin membrane-bound inhibitor homolog
• NCBI Official Synonym Full Names: BMP and activin membrane-bound inhibitor
• NCBI Official Symbol: Bambi
• NCBI Official Synonym Symbols: 2610003H06Rik
• NCBI Protein Information: BMP and activin membrane-bound inhibitor homolog
• UniProt Protein Name: BMP and activin membrane-bound inhibitor homolog
• Protein Family: BMP and activin membrane-bound inhibitor
• UniProt Gene Name: Bambi
• UniProt Synonym Gene Names: Nma

Uniprot Description

BAMBI: Negatively regulates TGF-beta signaling. Belongs to the BAMBI family.

Protein type: Inhibitor; Membrane protein, integral

Chromosomal Location of Human Ortholog: 18|18 A1

Cellular Component: cytoplasm; integral component of membrane; membrane; plasma membrane

Molecular Function: frizzled binding

Biological Process: cell migration; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; positive regulation of protein binding; positive regulation of transcription, DNA-templated; regulation of cell shape; transforming growth factor beta receptor signaling pathway