Rabbit anti-Human BAMBI Polyclonal Antibody | anti-BAMBI antibody

Anti-BAMBI Antibody, Rabbit Polyclonal

Cat. Num. AAA8104268

• Gene Names: BAMBI; NMA
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Protein A & Antigen Affinity
• Synonyms: BAMBI; Polyclonal Antibody; Anti-BAMBI Antibody; Rabbit Polyclonal; BAMBI/NMA Antibody; Rabbit PAb; Antigen Affinity Purified; BMP and activin membrane-bound inhibitor; Anti-NMA Antibody; anti-BAMBI antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human BAMBI/NMA
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-BAMBI antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:25,000-1:50,000
• Immunogen: Recombinant Human BAMBI protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human BAMBI (rh BAMBI). BAMBI specific IgG was purified by human BAMBI affinity chromatography .
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-BAMBI rabbit polyclonal antibody at 1:500 dilutionLane A: K562 Membrane LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:29 kDaObserved band size:26 kDa)

Related Product Information for anti-BAMBI antibody

BMP and activin membrane-bound inhibitor (BAMBI) is a transmembrane glycoprotein that is a pseudoreceptor of type 1 receptors. BAMBI structurally lacks intracellular serine/ threonine kinase domain but with an extracellular domain and a short cytoplasmic region that share sequence similarities with type 1 receptors, whose members have functions in signal transduction in various developing and pathological processes. BAMBI competes with the type 1 receptor, a receptor of the transforming growth factor-beta (TGF-beta), through functioning as negative regulators of TGF-beta by limiting the signaling range of the TGF-beta family during early embryogenesis. The expression of BAMBI can be induced by accumulated beta-catenin and BMP. The expression level of BAMBI was found aberrantly elevated in most colorectal and hepatocellular carcinomas relative to the corresponding non-cancerous tissues. It suggestes that beta-catenin and TGF-beta interfere growth arrest by inducing the expression of BAMBI, and this may contribute to colorectal and hepatocellular tumorigenesis.

References

Sekiya T, et al. (2003) Identification of BMP and Activin Membrane-bound Inhibitor (BAMBI), an Inhibitor of Transforming Growth Factor-Signaling, as a Target of the -Catenin Pathway in Colorectal Tumor Cells. The Journal of Biological Chemistry. 279:6840-6. Shi YG, et al. (2003) Mechanisms of TGF-Signaling from Cell Membrane to the Nucleus. Cell. 113(6): 685-700.Wanninger J, et al. (2011) Adiponectin induces the transforming growth factor decoy receptor BAMBI in human hepatocytes. FEBS Lett. 585(9):1338-44.

NCBI and Uniprot Product Information

• NCBI GI #: 6912534
• NCBI GeneID: 25805
• NCBI Accession #: NP_036474.1
• NCBI GenBank Nucleotide #: NM_012342.2
• UniProt Accession #: Q13145
• Molecular Weight: 29,108 Da
• NCBI Official Full Name: BMP and activin membrane-bound inhibitor homolog
• NCBI Official Synonym Full Names: BMP and activin membrane bound inhibitor
• NCBI Official Symbol: BAMBI
• NCBI Official Synonym Symbols: NMA
• NCBI Protein Information: BMP and activin membrane-bound inhibitor homolog
• UniProt Protein Name: BMP and activin membrane-bound inhibitor homolog
• Protein Family: BMP and activin membrane-bound inhibitor
• UniProt Gene Name: BAMBI
• UniProt Synonym Gene Names: NMA

NCBI Description

This gene encodes a transmembrane glycoprotein related to the type I receptors of the transforming growth factor-beta (TGF-beta) family, whose members play important roles in signal transduction in many developmental and pathological processes. The encoded protein however is a pseudoreceptor, lacking an intracellular serine/threonine kinase domain required for signaling. Similar proteins in frog, mouse and zebrafish function as negative regulators of TGF-beta, which has led to the suggestion that the encoded protein may function to limit the signaling range of the TGF-beta family during early embryogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

Negatively regulates TGF-beta signaling.