MAP1D Cell Lysate | METAP1D cell lysate
Human MAP1D Baculovirus-Insect cells Overexpression Lysate
Gene Names
METAP1D; MAP1D; MAP 1D; Metap1l; MetAP 1D
Applications
Western Blot
Synonyms
MAP1D; Human MAP1D Baculovirus-Insect cells Overexpression Lysate; Human MAP1D/Methionine Aminopeptidase 1D Insect Cell Lysate (WB positive control); methionyl aminopeptidase type 1D (mitochondrial); Human MAP 1D Overexpression Lysate; Human MAP1D Overexpression Lysate; Human MetAP 1D Overexpression Lysate; Human Metap1l Overexpression Lysate; METAP1D cell lysate
Host
Baculovirus-Insect cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
303aa
Sequence
Arg44-Ala335
Applicable Applications for METAP1D cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human MAP1D (NP_954697.1) (Arg 44-Ala 335) was expressed, with an initial Met at the N-terminus and a polyhistidine tag at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for METAP1D cell lysate
Methionine aminopeptidase 1D, also known as MAP1D, is a member of the peptidase M24A family. N-terminal methionine removal is an important cellular process required for proper biological activity, subcellular localization, and eventual degradation of many proteins. The enzymes that catalyze this reaction are called Methionine aminopeptidases (MAPs). MAP1D is overexpressed in colon cancer cell lines and colon tumors as compared to normal tissues (at protein level). Downregulation of MAP1D expression by shRNA in HCT-116 colon carcinoma cells reduces anchorage-independent growth in soft agar. MAP1D binds two cobalt ions per subunit. The true nature of the physiological cofactor is under debate. MAP1D is also active with zinc, manganese, or divalent ions. MAP1D removes the amino-terminal methionine from nascent proteins. It may also play an important role in colon tumorigenesis.
This Human MAP1D overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of MAP1D protein from the overexpression lysate was verified.
This Human MAP1D overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of MAP1D protein from the overexpression lysate was verified.
References
Serero A., et al., (2003), An unusual peptide deformylase features in the human mitochondrial N-terminal methionine excision pathway. J. Biol. Chem. 278:52953-52963.Leszczyniecka M., et al., (2006), MAP1D, a novel methionine aminopeptidase family member is overexpressed in colon cancer.Oncogene 25:3471-3478.Vaca Jacome A.S., et al., (2015), N-terminome analysis of the human mitochondrial proteome.Proteomics 15:2519-2524.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37,088 Da
NCBI Official Full Name
methionine aminopeptidase 1D, mitochondrial
NCBI Official Synonym Full Names
methionyl aminopeptidase type 1D (mitochondrial)
NCBI Official Symbol
METAP1D
NCBI Official Synonym Symbols
MAP1D; MAP 1D; Metap1l; MetAP 1D
NCBI Protein Information
methionine aminopeptidase 1D, mitochondrial; CDS of metAP-3 within PCR fragment; MAP 1D; metAP 1D; methionyl aminopeptidase type 1D, mitochondrial; peptidase M 1D
UniProt Protein Name
Methionine aminopeptidase 1D, mitochondrial
UniProt Gene Name
METAP1D
UniProt Entry Name
MAP12_HUMAN
NCBI Description
The N-terminal methionine excision pathway is an essential process in which the N-terminal methionine is removed from many proteins, thus facilitating subsequent protein modification. In mitochondria, enzymes that catalyze this reaction are celled methionine aminopeptidases (MetAps, or MAPs; EC 3.4.11.18) (Serero et al., 2003 [PubMed 14532271]).[supplied by OMIM, Mar 2008]
Uniprot Description
MAP1D: Removes the N-terminal methionine from nascent proteins. May play a role in colon tumorigenesis. Belongs to the peptidase M24A family.
Protein type: EC 3.4.11.18; Protease; Mitochondrial
Chromosomal Location of Human Ortholog: 2q31.1
Cellular Component: mitochondrion
Molecular Function: metalloexopeptidase activity; metal ion binding; aminopeptidase activity
Biological Process: peptidyl-methionine modification; proteolysis; N-terminal protein amino acid modification
Research Articles on METAP1D
Similar Products
Product Notes
The METAP1D metap1d (Catalog #AAA8114203) is a Cell Lysate produced from Baculovirus-Insect cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 303aa. AAA Biotech's MAP1D can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the METAP1D metap1d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Arg44-Ala3 35. It is sometimes possible for the material contained within the vial of "MAP1D, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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