CD3G recombinant protein

CD3G, 23-116aa, Human, His tag, E Coli (Denatured)

Cat. Num. AAA205489

• Gene Names: CD3G; T3G; IMD17; CD3-GAMMA
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: CD3G; 23-116aa; Human; His tag; E Coli (Denatured); CD3g molecule gamma (CD3-TCR complex); CD3g molecule; gamma (CD3-TCR complex); CD3-GAMMA; T3G; CD3G recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.4M urea, 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSQSIKGNH LVKVYDYQED GSVLLTCDAE AKNITWFKDG KMIGFLTEDK KKWNLGSNAK DPRGMYQCKG SQNKSKPLQV YYRMCQNCIE LNAATIS
• Sequence Length: 182

• Applicable Applications for CD3G recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for CD3G recombinant protein

The protein encoded by this gene is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. Defects in this gene are associated with T cell immunodeficiency. Recombinant human CD3G protein, fused to His-tag at N-terminus, was expressed in E Coli.

Product Categories/Family for CD3G recombinant protein

Immunology

References

Kjer-Nielsen L., et al. (2004) Proc. Natl. Acad. Sci. u.S.A. 101:7675-7680; Tao W.A., et al. (2005) Nat. Methods. 2:591-598;

NCBI and Uniprot Product Information

• NCBI GI #: 4557429
• NCBI GeneID: 917
• NCBI Accession #: NP_000064
• NCBI GenBank Nucleotide #: NM_000073.2
• UniProt Accession #: P09693; Q2HIZ6
• Molecular Weight: 13.1 kDa (117aa)
• NCBI Official Full Name: T-cell surface glycoprotein CD3 gamma chain
• NCBI Official Synonym Full Names: CD3g molecule
• NCBI Official Symbol: CD3G
• NCBI Official Synonym Symbols: T3G; IMD17; CD3-GAMMA
• NCBI Protein Information: T-cell surface glycoprotein CD3 gamma chain
• UniProt Protein Name: T-cell surface glycoprotein CD3 gamma chain
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: CD3G
• UniProt Synonym Gene Names: T3G

NCBI Description

The protein encoded by this gene is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. Defects in this gene are associated with T cell immunodeficiency. [provided by RefSeq, Jul 2008]

Uniprot Description

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition to this role of signal transduction in T-cell activation, CD3G plays an essential role in the dynamic regulation of TCR expression at the cell surface (PubMed:8187769). Indeed, constitutive TCR cycling is dependent on the di-leucine-based (diL) receptor-sorting motif present in CD3G.