Smad5 cell lysate
Mouse Smad5 Baculovirus-Insect cells Overexpression Lysate
Gene Names
Smad5; Dwf-C; Madh5; MusMLP; AI451355; 1110051M15Rik
Applications
Western Blot
Synonyms
Smad5; Mouse Smad5 Baculovirus-Insect cells Overexpression Lysate; Mouse Smad5 Insect Cell Lysate (WB positive control); SMAD family member 5; Mouse 1110051M15Rik Overexpression Lysate; Mouse AI451355 Overexpression Lysate; Mouse Dwf-C Overexpression Lysate; Mouse Madh5 Overexpression Lysate; Mouse MusMLP Overexpression Lysate; Smad5 cell lysate
Host
Baculovirus-Insect cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
701aa
Sequence
Thr2-Ser465
Applicable Applications for Smad5 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse SMAD5 (P97454) (Thr2-Ser465) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for Smad5 cell lysate
SMAD5 is a member of the SMAD family. Members of this family mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD5 is involved in the TGF-beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. It is also involved in cell signalling and modulates signals of bone morphogenetic proteins (BMP's). The binding of ligands causes the oligomerization and phosphorylation of the SMAD5 protein. SMAD5 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase.
This Mouse Smad5 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of Smad5 protein from the overexpression lysate was verified.
This Mouse Smad5 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of Smad5 protein from the overexpression lysate was verified.
Product Categories/Family for Smad5 cell lysate
References
Vinayagam A. et al., 2011, Sci Signal. 4 (189): rs8. Sangadala S. et al., 2007, J Biomol Struct Dyn. 25 (1): 11-23. Riggins GJ. et al., 1996, Nat Genet. 13 (3): 347-9.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52,172 Da
NCBI Official Full Name
mothers against decapentaplegic homolog 5
NCBI Official Synonym Full Names
SMAD family member 5
NCBI Official Symbol
Smad5
NCBI Official Synonym Symbols
Dwf-C; Madh5; MusMLP; AI451355; 1110051M15Rik
NCBI Protein Information
mothers against decapentaplegic homolog 5; Smad 5; mSmad5; dwarfin-C; MAD homolog 5; mothers against DPP homolog 5
UniProt Protein Name
Mothers against decapentaplegic homolog 5
Protein Family
UniProt Gene Name
Smad5
UniProt Synonym Gene Names
Madh5; Msmad5; MAD homolog 5; Mothers against DPP homolog 5; Dwf-C; SMAD 5; Smad5; mSmad5
UniProt Entry Name
SMAD5_MOUSE
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Product Notes
The Smad5 smad5 (Catalog #AAA8116834) is a Cell Lysate produced from Baculovirus-Insect cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 701aa. AAA Biotech's Smad5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the Smad5 smad5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Thr2-Ser46 5. It is sometimes possible for the material contained within the vial of "Smad5, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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