TGFBR2 active protein

TGFBR2 Protein, Human, Recombinant (ECD, His Tag)

Cat. Num. AAA8120228

• Gene Names: TGFBR2; AAT3; FAA3; LDS2; MFS2; RIIC; LDS1B; LDS2B; TAAD2; TGFR-2; TGFbeta-RII
• Purity: >90% as determined by SDS-PAGE
• Synonyms: TGFBR2; TGFBR2 Protein; Human; Recombinant (ECD; His Tag); Human TGFBR2 Protein (ECD; AAT3 Protein; FAA3 Protein; LDS1B Protein; LDS2 Protein; LDS2B Protein; MFS2 Protein; RIIC Protein; TAAD2 Protein; TGFbeta-RII Protein; TGFR-2 Protein; transforming growth factor; beta receptor II (70/80kDa); TGFBR2 active protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM PBS, 150mM NaCl, 10% glycerol, 0.5mM TCEP, pH7.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gln166

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Thr 23
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human TGFBR2 (NP_003233.4)(Met1-Gln166) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Measured by its binding ability in a functional ELISA. Immobilized TGFBR2h (1-166Q) at 10ug/mL (100uL/well) can bind TGFB1-His/Biotin. The EC50 of human TGFB1-His/Biotin is 130-300ng/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its binding ability in a functional ELISA. Immobilized TGFBR2h (1-166Q) at 10 ug/mL (100 uL/well) can bind TGFB1-His/Biotin, the EC50 of human TGFB1-His/Biotin is 0.50-1.20 ug/mL.)

Related Product Information for TGFBR2 active protein

Background: TGFBR2 is a member of the Ser/Thr protein kinase family and the TGFB receptor subfamily. It is a transmembrane protein. TGFBR2 is comprised of a C-terminal protein kinase domain and an N-terminal ectodomain. The ectodomain consists of a compact fold containing nine beta-strands and a single helix stabilized by a network of six intra strand disulfide bonds. The folding topology includes a central five-stranded antiparallel beta-sheet, eight-residues long at its centre, covered by a second layer consisting of two segments of two-stranded antiparallel beta-sheets. TGFBR2 has a protein kinase domain, forms a heterodimeric complex with another receptor protein, and binds TGF-beta. This receptor/ligand complex phosphorylates proteins, which then enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation. Mutations in TGFBR2 gene have been associated with Marfan syndrome, Loeys-Deitz Aortic Aneurysm Syndrome, and the development of various types of tumors. TGFBR2 attenuates the biological activities of TGF-beta in colorectal cancer. TGFBR2 expression is increased in oral squamous cell carcinoma cells. Its expression is decreased by IL-1beta while inducing Sp3 via NFkappaB. TGFB2 and TGFBR2 are involved in the antiestrogenic activity.

References

Yu Y, et al. (2012) MicroRNA-21 induces stemness by downregulating transforming growth factor beta receptor 2 (TGFbetaR2) in colon cancer cells. Carcinogenesis. 33(1):68-76. Shima K, et al. (2011) TGFBR2 and BAX mononucleotide tract mutations, microsatellite instability, and prognosis in 1072 colorectal cancers. PLoS One. 6(9):e25062.Biros E, et al. (2011) Meta-analysis of the association between single nucleotide polymorphisms in TGF-beta receptor genes and abdominal aortic aneurysm. Atherosclerosis. 219(1):218-23.

NCBI and Uniprot Product Information

• NCBI GI #: 67782324
• NCBI GeneID: 7048
• NCBI Accession #: NP_003233.4
• NCBI GenBank Nucleotide #: NM_003242.5
• UniProt Accession #: P37173; Q15580; Q6DKT6; Q99474; B4DTV5
• Molecular Weight: 67,457 Da
• NCBI Official Full Name: TGF-beta receptor type-2 isoform B
• NCBI Official Synonym Full Names: transforming growth factor, beta receptor II (70/80kDa)
• NCBI Official Symbol: TGFBR2
• NCBI Official Synonym Symbols: AAT3; FAA3; LDS2; MFS2; RIIC; LDS1B; LDS2B; TAAD2; TGFR-2; TGFbeta-RII
• NCBI Protein Information: TGF-beta receptor type-2; tbetaR-II; TGF-beta type II receptor; TGF-beta receptor type IIB; transforming growth factor beta receptor type IIC; transforming growth factor, beta receptor II beta; transforming growth factor, beta receptor II alpha; transform
• UniProt Protein Name: TGF-beta receptor type-2
• Protein Family: TGF-beta receptor
• UniProt Gene Name: TGFBR2
• UniProt Synonym Gene Names: TGFR-2; TGF-beta receptor type II; TbetaR-II
• UniProt Entry Name: TGFR2_HUMAN

NCBI Description

This gene encodes a member of the Ser/Thr protein kinase family and the TGFB receptor subfamily. The encoded protein is a transmembrane protein that has a protein kinase domain, forms a heterodimeric complex with another receptor protein, and binds TGF-beta. This receptor/ligand complex phosphorylates proteins, which then enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation. Mutations in this gene have been associated with Marfan Syndrome, Loeys-Deitz Aortic Aneurysm Syndrome, and the development of various types of tumors. Alternatively spliced transcript variants encoding different isoforms have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

TGFBR2: a TKL kinase of the serine/threonine-protein kinase receptor (STKR) family. R1 and R2 TGF-beta receptors dimerize after binding TGF-beta at the cell surface. Binds to DAXX. Defects can cause esophageal cancer.

Protein type: EC 2.7.11.30; Oncoprotein; Membrane protein, integral; Kinase, protein; Protein kinase, TKL; Protein kinase, Ser/Thr (receptor); TKL group; STKR family; Type2 subfamily

Chromosomal Location of Human Ortholog: 3p22

Cellular Component: plasma membrane; integral to membrane; caveola; cytosol; receptor complex; external side of plasma membrane

Molecular Function: transforming growth factor beta receptor activity; protein binding; glycosaminoglycan binding; transforming growth factor beta receptor activity, type II; transforming growth factor beta binding; metal ion binding; mitogen-activated protein kinase kinase kinase binding; SMAD binding; ATP binding; transmembrane receptor protein serine/threonine kinase activity; receptor signaling protein serine/threonine kinase activity

Biological Process: lens development in camera-type eye; wound healing; apoptosis; heart development; positive regulation of smooth muscle cell proliferation; myeloid dendritic cell differentiation; gastrulation; palate development; positive regulation of tolerance induction to self antigen; protein amino acid phosphorylation; negative regulation of cardiac muscle cell proliferation; transforming growth factor beta receptor signaling pathway; positive regulation of mesenchymal cell proliferation; positive regulation of cell proliferation; response to glucose stimulus; positive regulation of NK T cell differentiation; vasculogenesis; positive regulation of T cell tolerance induction; response to nutrient; aging; response to drug; receptor-mediated endocytosis; blood vessel development; smoothened signaling pathway; organ regeneration; positive regulation of skeletal muscle regeneration; in utero embryonic development; embryonic hemopoiesis; common-partner SMAD protein phosphorylation; peptidyl-threonine phosphorylation; embryonic cranial skeleton morphogenesis; regulation of cell proliferation; patterning of blood vessels; peptidyl-serine phosphorylation; positive regulation of angiogenesis; gut development; regulation of gene expression; response to mechanical stimulus; response to estrogen stimulus; positive regulation of B cell tolerance induction; activation of protein kinase activity; brain development; negative regulation of transforming growth factor beta receptor signaling pathway; embryo implantation

Disease: Colorectal Cancer, Hereditary Nonpolyposis, Type 6; Loeys-dietz Syndrome 2; Esophageal Cancer