Rabbit anti-Human POU2AF1 Polyclonal Antibody | anti-POU2AF1 antibody

POU2AF1, NT (POU2AF1, OBF1, POU domain class 2-associating factor 1, B-cell-specific coactivator OBF-1, BOB-1, OCA-B, OCT-binding factor 1)

Cat. Num. AAA647020

• Gene Names: POU2AF1; BOB1; OBF1; OCAB; OBF-1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: POU2AF1; Polyclonal Antibody; NT (POU2AF1; OBF1; POU domain class 2-associating factor 1; B-cell-specific coactivator OBF-1; BOB-1; OCA-B; OCT-binding factor 1); Anti -POU2AF1; anti-POU2AF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-POU2AF1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Flow Cytometry: 1:10-50; Immunohistochemistry: 1:10-50
• Immunogen: POU2AF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7~36 amino acids from the N-terminal region of human POU2AF1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of MBS647020 in Ramos cell line lysates (35ug/lane). POU2AF1 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(POU2AF1 Antibody (N-term) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunohistochemistry (IHC)

(POU2AF1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Related Product Information for anti-POU2AF1 antibody

POU2AF1 is transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.

Product Categories/Family for anti-POU2AF1 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 167900478
• NCBI GeneID: 5450
• NCBI Accession #: NP_006226.2
• NCBI GenBank Nucleotide #: NM_006235.2
• UniProt Accession #: Q16633; Q14983; B2R8Z9; AAB34937
• Molecular Weight: 27,436 Da
• NCBI Official Full Name: POU domain class 2-associating factor 1
• NCBI Official Synonym Full Names: POU class 2 associating factor 1
• NCBI Official Symbol: POU2AF1
• NCBI Official Synonym Symbols: BOB1; OBF1; OCAB; OBF-1
• NCBI Protein Information: POU domain class 2-associating factor 1; BOB-1; OCA-B; OCT-binding factor 1; B-cell-specific coactivator OBF-1; POU domain, class 2, associating factor 1
• UniProt Protein Name: POU domain class 2-associating factor 1
• Protein Family: POU domain class 2-associating factor
• UniProt Gene Name: POU2AF1
• UniProt Synonym Gene Names: OBF1
• UniProt Entry Name: OBF1_HUMAN

Uniprot Description

Function: Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.

Subcellular location: Nucleus.

Tissue specificity: B-cell specific.

Post-translational modification: Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation

Probable. Ref.8 Ref.9

Involvement in disease: A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6.

Sequence similarities: Belongs to the POU2AF1 family.