IL-9 active protein

Human IL-9 Protein, His Tag

Cat. Num. AAA9718705

• Gene Names: IL9; P40; HP40; IL-9
• Purity: >97% as determined by SDS-PAGE
• Synonyms: IL-9; Human IL-9 Protein; His Tag; Recombinant Human IL9 Protein; His Tag (Interleukin-9) expressed in Baculovirus-Insect Cells; HP40; P40; Interleukin-9; IL9; IL-9 active protein

• Host: Baculovirus
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4
• Sequence: Amino acid sequence derived from human IL9 (NP_000581.1) (Gln 19-Il4 144) was expressed and purified, fused with a polyhistidine tag at the C-terminus.
• Sequence Length: 144

• Species: Human
• Endotoxin: The Endotoxin level is less than 1.0 EU per mug of the protein as determined by the LAL method
• Protein Length: The recombinant human IL9 consists of 136 amino acids and predicts a molecular mass of 15.5 kDa. rhIL9 migrates as multiple bands with the molecular mass of 18-25 kDa band in SDS-PAGE under reducing conditions due to different glycosylation.
• Activity: Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 0.5-3 ng/mL.
• Usage Notes: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL9 protein, His Tag in sterile ddH2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Item should be stored desiccated below -20 degree C. Upon reconstitution, the protein should be stored at 4 degree C between 2-7 days and for future use below -20 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

SDS-Page

(Fig.SDS-PAGE analysis of Human IL-9 protein, His Tag.)

Related Product Information for IL-9 active protein

Interleukin 9, also known as IL-9, is a cytokine (cell signalling molecule) belonging to the group of interleukins. IL-9 is a cytokine that acts as a regulator of a variety of hematopoietic cells. This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL-9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness. IL-9 is a key molecule that affects differentiation of TH17 cells and Treg function. IL-9 predominantly produced by TH17 cells, synergizes with TGF-beta1 to differentiate naïve CD4+ T cells into TH17 cells, while IL-9 secretion by TH17 cells is regulated by IL-23. Interestingly, IL-9 enhances the suppressive functions of FoxP3+ CD4+ Treg cells in vitro, and absence of IL-9 signaling weakens the suppressive activity of nTregs in vivo, leading to an increase in effector cells and worsening of experimental autoimmune encephalomyelitis. The mechanism of IL-9 effects on TH17 and Tregs is through activation of STAT3 and STAT5 signaling. Our findings highlight a role of IL-9 as a regulator of pathogenic versus protective mechanisms of immune responses.

NCBI and Uniprot Product Information

• NCBI GI #: 10834980
• NCBI GeneID: 3578
• NCBI Accession #: NP_000581.1
• NCBI GenBank Nucleotide #: NP_000581.1
• UniProt Accession #: P15248
• Molecular Weight: 15.5 kDa
• NCBI Official Full Name: interleukin-9
• NCBI Official Synonym Full Names: interleukin 9
• NCBI Official Symbol: IL9
• NCBI Official Synonym Symbols: P40; HP40; IL-9
• NCBI Protein Information: interleukin-9

NCBI Description

The protein encoded by this gene is a cytokine that acts as a regulator of a variety of hematopoietic cells. This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. The gene encoding this cytokine has been identified as a candidate gene for asthma. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness. [provided by RefSeq, Jul 2008]