Rabbit anti-Mouse IL-9 Polyclonal Antibody | anti-IL9 antibody

Anti-IL-9 Antibody, Rabbit Polyclonal

Cat. Num. AAA8100247

• Gene Names: Il9; P40; Il-9
• Reactivity: Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: IL-9; Polyclonal Antibody; Anti-IL-9 Antibody; Rabbit Polyclonal; IL9 Antibody; Rabbit PAb; Antigen Affinity Purified; interleukin 9; Anti-Il-9 Antibody; Anti-P40 Antibody; anti-IL9 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse IL9
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-IL9 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:500-1:1000; IHC-P: 1:5000-1:25,000
• Immunogen: A synthetic peptide corresponding to the N-terminus of the mouse IL9
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with A synthetic peptide corresponding to the N-terminus of the mouse IL9, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of mouse IL9 in mouse spleen (from 2 donors) with rabbit polyclonal antibody at 1:25000 dilution, formalin-fixed paraffin embedded sections.)

Western Blot (WB)

(Anti-IL9 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:15 kDaObserved band size:17 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-IL9 antibody

Interleukin 9, also known as IL-9, is a cytokine (cell signaling molecule) belonging to the group of interleukins. IL-9 is a cytokine that acts as a regulator of a variety of hematopoietic cells. This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL-9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness. IL-9 is a key molecule that affects the differentiation of TH17 cells and Treg function. IL-9 predominantly produced by TH17 cells synergizes with TGF-beta1 to differentiate naive CD4+ T cells into TH17 cells, while IL-9 secretion by TH17 cells is regulated by IL-23. Interestingly, IL-9 enhances the suppressive functions of FoxP3+ CD4+ Treg cells in vitro, and the absence of IL-9 signaling weakens the suppressive activity of nTregs in vivo, leading to an increase in effector cells and worsening of experimental autoimmune encephalomyelitis. The mechanism of IL-9 effects on TH17 and Tregs is through activation of STAT3 and STAT5 signaling. Our findings highlight the role of IL-9 as a regulator of pathogenic versus protective mechanisms of immune responses.

Product Categories/Family for anti-IL9 antibody

Common G-Chain signaling pathway

References

Elyaman W, et al. (2009) IL-9 induces differentiation of TH17 cells and enhances function of FoxP3+ natural regulatory T cells. Proc Natl Acad Sci U S A. 106(31): 12885-90.Dong Q, et al. (1999) IL-9 induces chemokine expression in lung epithelial cells and baseline airway eosinophilia in transgenic mice. Eur J Immunol. 29(7): 2130-9.Kimura Y, et al. (1995) Sharing of the IL-2 receptor gamma chain with the functional IL-9 receptor complex. Int Immunol. 7(1): 115-20.

NCBI and Uniprot Product Information

• NCBI GI #: 6680437
• NCBI GeneID: 16198
• NCBI Accession #: NP_032399.1
• NCBI GenBank Nucleotide #: NM_008373.1
• UniProt Accession #: P15247
• Molecular Weight: 16,075 Da
• NCBI Official Full Name: interleukin-9
• NCBI Official Synonym Full Names: interleukin 9
• NCBI Official Symbol: Il9
• NCBI Official Synonym Symbols: P40; Il-9
• NCBI Protein Information: interleukin-9; T-cell growth factor P40; cytokine P40
• UniProt Protein Name: Interleukin-9
• Protein Family: Interleukin
• UniProt Gene Name: Il9
• UniProt Synonym Gene Names: IL-9
• UniProt Entry Name: IL9_MOUSE

Uniprot Description

IL9: Supports IL-2 independent and IL-4 independent growth of helper T-cells. Belongs to the IL-7/IL-9 family.

Protein type: Cytokine; Secreted; Secreted, signal peptide

Cellular Component: extracellular space; extracellular region

Molecular Function: growth factor activity; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; interleukin-9 receptor binding; cytokine activity

Biological Process: immune response; positive regulation of cell growth; positive regulation of interleukin-5 biosynthetic process