IL-6R alpha active protein

Recombinant IL-6R alpha

Cat. Num. AAA201184

• Gene Names: IL6R; IL6Q; gp80; CD126; IL6RA; IL6RQ; IL-6RA; IL-6R-1
• Applications: SDS-Page
• Purity: > 90% by SDS - PAGE
• Synonyms: IL-6R alpha; Recombinant IL-6R alpha; Interleukin-6 receptor subunit alpha isoform 1; IL6R; CD126; gp80; IL-6R-1; IL-6RA; IL6Q; IL6RA; IL6RQ; IL-6R alpha active protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS - PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 20-365aa
• Sequence: LAPRRCP AQEVARGVLT SLPGDSVTLT CPGVEPEDNA TVHWVLRKPA AGSHPSRWAGMGRRLLLRSV QLHDSGNYSC YRAGRPAGTV HLLVDVPPEE PQLSCFRKSP LSNVVCEWGPRSTPSLTTKA VLLVRKFQNS PAEDFQEPCQ YSQESQKFSC QLAVPEGDSS FYIVSMCVASSVGSKFSKTQ TFQGCGILQP DPPANITVTA VARNPRWLSV TWQDPHSWNS SFYRLRFELRYRAERSKTFT TWMVKDLQHH CVIHDAWSGL RHVVQLRAQE EFGQGEWSEW SPEAMGTPWTESRSPPAENE VSTPMQALTT NKDDDNILFR DSANATSLPV QDSSSVPLP
• Sequence Length: 53

• Applicable Applications for IL-6R alpha active protein: SDS-PAGE; Bioactivity
• Tag: His-Tag
• Biological Activity: Measured by its ability to inhibit proliferation using M1 mouse myeloid leukemia cells. The ED50 range 20 ng/ml with Human IL-6.
• Predicted nterm: Ala
• Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
• Preparation and Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoidrepeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)

Activity

(Human IL-6 R alpha inhibits Human IL-6 induced cell proliferation in the M1 mouse myeloid leukemia cells. The ED50 range 20 ng/ml.)

Related Product Information for IL-6R alpha active protein

IL-6R alpha, also known as interleukin-6 receptor subunit alpha isoform 1, is a type I cytokine receptor. It is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in immune response. It has a modular build of several immunoglobulin-like and fibronectin type III-like domains. The low concentration of a soluble form of IL-6 receptor (sIL-6R) acts as an agonist of IL-6 activity. In the IL-6R/CD126/IL6R system, both a membrane-bound IL-6R and a sIL-6R protein are able to mediate IL-6 signals into the cells through the interaction of gp130. It is implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. Recombinant human IL-6R alpha, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for IL-6R alpha active protein

Hormones & Cytokines; Immunology

References

Sugita T., et al. (1990) J Exp Med. 171:2001-2009.;;Yamasaki K., et al. (1988) Science. 241:825-828.

NCBI and Uniprot Product Information

• NCBI GI #: 633863
• NCBI GeneID: 3570
• UniProt Accession #: P08887
• Molecular Weight: 39.6kDa (355aa)
• NCBI Official Full Name: Soluble interleukin-6 receptor, partial
• NCBI Official Synonym Full Names: interleukin 6 receptor
• NCBI Official Symbol: IL6R
• NCBI Official Synonym Symbols: IL6Q; gp80; CD126; IL6RA; IL6RQ; IL-6RA; IL-6R-1
• NCBI Protein Information: interleukin-6 receptor subunit alpha
• UniProt Protein Name: Interleukin-6 receptor subunit alpha
• UniProt Gene Name: IL6R
• UniProt Synonym Gene Names: IL-6 receptor subunit alpha; IL-6R subunit alpha; IL-6R-alpha; IL-6RA; gp80
• UniProt Entry Name: IL6RA_HUMAN

NCBI Description

This gene encodes a subunit of the interleukin 6 (IL6) receptor complex. Interleukin 6 is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in the immune response. The IL6 receptor is a protein complex consisting of this protein and interleukin 6 signal transducer (IL6ST/GP130/IL6-beta), a receptor subunit also shared by many other cytokines. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. Alternatively spliced transcript variants encoding distinct isoforms have been reported. A pseudogene of this gene is found on chromosome 9.[provided by RefSeq, May 2011]

Uniprot Description

IL6R: Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis. Belongs to the type I cytokine receptor family. Type 3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: extracellular space; basolateral plasma membrane; apical plasma membrane; extracellular region; plasma membrane; interleukin-6 receptor complex

Molecular Function: protein binding; interleukin-6 binding; protein homodimerization activity; enzyme binding; interleukin-6 receptor binding; ciliary neurotrophic factor receptor activity; interleukin-6 receptor activity

Biological Process: positive regulation of smooth muscle cell proliferation; negative regulation of collagen biosynthetic process; cytokine and chemokine mediated signaling pathway; neutrophil mediated immunity; positive regulation of interleukin-6 production; positive regulation of leukocyte chemotaxis; defense response to Gram-negative bacterium; endocrine pancreas development; positive regulation of chemokine production; activation of NF-kappaB transcription factor; positive regulation of tyrosine phosphorylation of Stat3 protein; monocyte chemotaxis; positive regulation of osteoblast differentiation; positive regulation of peptidyl-tyrosine phosphorylation; defense response to Gram-positive bacterium; positive regulation of MAPKKK cascade; response to cytokine stimulus; positive regulation of cell proliferation; acute-phase response; negative regulation of interleukin-8 production; hepatic immune response

Disease: Interleukin 6, Serum Level Of, Quantitative Trait Locus; Soluble Interleukin-6 Receptor, Serum Level Of, Quantitative Trait Locus