B2M / Beta-2-microglobulin Recombinant Protein | B2M recombinant protein

Recombinant Rat B2M / Beta-2-microglobulin Protein (His tag)

Cat. Num. AAA2547295

• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: B2M / Beta-2-microglobulin; Recombinant Rat B2M / Beta-2-microglobulin Protein (His tag); B2M; B2M recombinant protein

• Host: Human Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 119

• Application Notes: The secreted recombinant rat B2M comprises 110 amino acids and predicts a molecular mass of 13 kDa. The apparent molecular mass of the rat B2M is approximately 15 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Ile 21
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

Related Product Information for B2M recombinant protein

Background: B2M, also known as beta2-Microglobulin or CDABP0092, is a component of MHC class I molecules found expression in all nucleated cells (excludes red blood cells). The major function of MHC class I moleculesis is to display fragments of proteins from within the cell to T-cells and cells containing foreign proteins will be attacked. B2M (beta2-Microglobulin) is a low molecular weight protein. It was demonstrated that B2M (beta2-Microglobulin) was localized in the membranes of nucleated cells and was found to be associated with HL-A antigens.B2M (beta2- Microglobulin) is present in free form in various body fluids and as a subunit of histocompatibility antigens on cell surfaces lateral to thealpha3 chain. Unlikealpha3, beta2 has no transmembrane region. Directly above beta2 lies the alpha1 chain, which itself is lateral to the alpha2. In the absence of B2M (beta2 microglobulin), very limited amounts of MHC class I (classical and non-classical) molecules can be detected on the surface. In the absence of MHC class I, CD8 T cells, a subset of T cells involved in the development of acquired immunity cannot develop. Low levels of B2M (beta2 microglobulin) can indicate non-progression of HIV.

Description: A DNA sequence encoding the rat B2M (P07151) (Met 1-Met 119) was expressed, fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 7549746
• NCBI GeneID: 24223
• NCBI Accession #: NP_036644.1
• NCBI GenBank Nucleotide #: NM_012512.2
• UniProt Accession #: P07151
• Molecular Weight: 13,720 Da
• NCBI Official Full Name: beta-2-microglobulin
• NCBI Official Synonym Full Names: beta-2 microglobulin
• NCBI Official Symbol: B2m
• NCBI Protein Information: beta-2-microglobulin
• UniProt Protein Name: Beta-2-microglobulin
• Protein Family: Beta-2-microglobulin
• UniProt Gene Name: B2m

NCBI Description

mouse homolog plays a role in the assembly and surface expression of MHC class I molecules [RGD, Feb 2006]

Uniprot Description

B2M: Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Heterodimer of an alpha chain and a beta chain. Beta-2- microglobulin is the beta-chain of major histocompatibility complex class I molecules. Polymers of beta 2-microglobulin can be found in tissues from patients on long-term hemodialysis. Belongs to the beta-2-microglobulin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3q35

Cellular Component: cytoplasm; external side of plasma membrane; extracellular space; focal adhesion; Golgi apparatus; membrane; plasma membrane

Molecular Function: glycoprotein binding; identical protein binding

Biological Process: antibacterial humoral response; antigen processing and presentation of endogenous peptide antigen via MHC class I; antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent; defense response to Gram-negative bacterium; defense response to Gram-positive bacterium; ferric iron import; innate immune response; iron ion homeostasis; iron ion transport; positive regulation of protein binding; positive regulation of receptor-mediated endocytosis; positive regulation of T cell cytokine production; positive regulation of T cell mediated cytotoxicity; protein refolding; regulation of erythrocyte differentiation; response to cadmium ion; response to drug; response to molecule of bacterial origin; retinal homeostasis; T cell differentiation in the thymus