Immunohistochemistry (IHC)
(Immunochemical staining of mouse B2M in mouse skin with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Rabbit anti-Mouse Beta-2 microglobulin Polyclonal Antibody | anti-B2M antibody
Anti-Beta-2 microglobulin Antibody, Rabbit Polyclonal
Gene Names
B2m; Ly-m11; beta2m; beta2-m
Reactivity
Mouse
Applications
ELISA, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
Beta-2 microglobulin; Polyclonal Antibody; Anti-Beta-2 microglobulin Antibody; Rabbit Polyclonal; B2M/Beta-2-microglobulin Antibody; Rabbit PAb; Antigen Affinity Purified; beta-2 microglobulin; Anti-beta2-m Antibody; Anti-beta2m Antibody; Anti-Ly-m11 Antibody; anti-B2M antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse B2M/Beta-2-microglobulin
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-B2M antibody
ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
ELISA: 1:5000-1:10,000
IHC-P: 1:500-1:2000
IHC-P: 1:500-1:2000
Immunogen
Recombinant Mouse B2M/Beta-2-microglobulin protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Mouse B2M/Beta-2-microglobulin (rM B2M/Beta-2-microglobulin). B2M/Beta-2-microglobulin specific IgG was purified by Mouse B2M/Beta-2-microglobulin affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of mouse B2M in mouse skin with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse B2M in mouse skin with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Related Product Information for anti-B2M antibody
B2M, also known as beta2-Microglobulin or CDABP0092, is a component of MHC class I molecules found expression in all nucleated cells (excludes red blood cells). The major function of MHC class I moleculesis is to display fragments of proteins from within the cell to T-cells and cells containing foreign proteins will be attacked. B2M (beta2-Microglobulin) is a low molecular weight protein. It was demonstrated that B2M (beta2-Microglobulin) was localized in the membranes of nucleated cells and was found to be associated with HL-A antigens. B2M (beta2-Microglobulin) is present in free form in various body fluids and as a subunit of histocompatibility antigens on cell surfaces lateral to the alpha3 chain. Unlike alpha3, beta2 has no transmembrane region. Directly above beta2 lies the alpha1 chain, which itself is lateral to the alpha2. In the absence of B2M (beta2 microglobulin), very limited amounts of MHC class I (classical and non-classical) molecules can be detected on the surface. In the absence of MHC class I, CD8 T cells, a subset of T cells involved in the development of acquired immunity cannot develop. Low levels of B2M (beta2 microglobulin) can indicate non-progression of HIV.
References
Poulik MD, et al. (1979) Beta 2-Microglobulin: methods and clinical applications. CRC Ctit Rev Clin Lab Sci. 10(3): 225-45.Poulik MD, et al. (1975) Beta2-Microglobulins. Contemp Top Mol Immunol. 4: 157-204.Berggard I. (1976) Beta2-Microglobulins: isolation, properties, and distribution. Fed Proc. 35(5): 1167-70.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
13,779 Da
NCBI Official Full Name
beta-2-microglobulin
NCBI Official Synonym Full Names
beta-2 microglobulin
NCBI Official Symbol
B2m
NCBI Official Synonym Symbols
Ly-m11; beta2m; beta2-m
NCBI Protein Information
beta-2-microglobulin
UniProt Protein Name
Beta-2-microglobulin
Protein Family
UniProt Gene Name
B2m
Uniprot Description
Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
Research Articles on B2M
Similar Products
Product Notes
The B2M b2m (Catalog #AAA8112197) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Beta-2 microglobulin Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Beta-2 microglobulin can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P). ELISA: 1:5000-1:10,000 IHC-P: 1:500-1:2000. Researchers should empirically determine the suitability of the B2M b2m for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Beta-2 microglobulin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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