Mouse Heparin binding protein ELISA Kit | AZU/HBP elisa kit

Mouse Heparin binding protein ELISA Kit

Cat. Num. AAA731437

• Reactivity: Mouse
• Synonyms: Heparin binding protein; Mouse Heparin binding protein ELISA Kit; AZU/HBP elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This HBP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse HBP. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AZU/HBP elisa kit

Principle of the assay: HBP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HBP antibody and an HBP-HRP conjugate. The assay sample and buffer are incubated together with HBP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HBP concentration since HBP from samples and HBP-HRP conjugate compete for the anti-HBP antibody binding site. Since the number of sites is limited, as more sites are occupied by HBP from the sample, fewer sites are left to bind HBP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HBP concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 183951
• Molecular Weight: 26,264 Da
• NCBI Official Full Name: heparin binding protein
• UniProt Protein Name: Fibroblast growth factor-binding protein 1
• UniProt Gene Name: FGFBP1
• UniProt Synonym Gene Names: FGFBP; HBP17; FGF-BP; FGF-BP1; FGF-binding protein 1; FGFBP-1; 17 kDa HBGF-binding protein; HBp17
• UniProt Entry Name: FGFP1_HUMAN

NCBI Description

This gene encodes a secreted fibroblast growth factor carrier protein. The encoded protein plays a critical role in cell proliferation, differentiation and migration by binding to fibroblast growth factors and potentiating their biological effects on target cells. The encoded protein may also play a role in tumor growth as an angiogenic switch molecule, and expression of this gene has been associated with several types of cancer including pancreatic and colorectal adenocarcinoma. A pseudogene of this gene is also located on the short arm of chromosome 4. [provided by RefSeq, Nov 2011]

Uniprot Description

FGFBP1: Acts as a carrier protein that release fibroblast- binding factors (FGFs) from the extracellular matrix (EM) storage and thus enhance the mitogenic activity of FGFs. Enhances FGF2 signaling during tissue repair, angiogenesis and in tumor growth. Belongs to the fibroblast growth factor-binding protein family.

Chromosomal Location of Human Ortholog: 4p15.32

Cellular Component: extracellular space; cell surface; plasma membrane

Molecular Function: heparin binding; protein binding; fibroblast growth factor binding

Biological Process: negative regulation of cell proliferation; cell-cell signaling; positive regulation of cell proliferation; positive regulation of fibroblast growth factor receptor signaling pathway; signal transduction