Human TYRO3 ELISA Kit | TYRO3 elisa kit

Human TYRO3 ELISA Kit

Cat. Num. AAA8248414

• Gene Names: TYRO3; BYK; Dtk; RSE; Rek; Sky; Tif; Etk-2
• Reactivity: Human
• Synonyms: TYRO3; Human TYRO3 ELISA Kit; BYK; DTK; RSE; SKY; TIF; Tyrosine-protein kinase receptor TYRO3; Tyrosine-protein kinase BYK; Tyrosine-protein kinase DTK; Tyrosine-protein kinase RSE; Tyrosine-protein kinase SKY; Tyrosine-protein kinase TIF; TYRO3 elisa kit

• Reactivity: Human
• Specificity: The Human TYRO3 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human TYRO3 proteins within the range of 125 pg/ml - 8000 pg/ml.
• Sequence Length: 845

• Samples: Cell Culture Supernates, Serum, Plasma
• Sensitivity: <10 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TYRO3 elisa kit

Principle of the Assay: The Human TYRO3 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TYRO3 in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Human TYRO3 coated on a 96-well plate. Standards and samples are pipetted into the wells and TYRO3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TYRO3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TYRO3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Tyrosine-protein kinase receptor TYRO3 is an enzyme that in humans is encoded by the TYRO3 gene. The gene is part of a 3-member transmembrane receptor kinase receptor family with a processed pseudogene distal on chromosome 15. The encoded protein is activated by the products of the growth arrest-specific gene 6 and protein S genes and is involved in controlling cell survival and proliferation, spermatogenesis, immunoregulation and phagocytosis. The encoded protein has also been identified as a cell entry factor for Ebola and Marburg viruses.

NCBI and Uniprot Product Information

• NCBI GI #: 1057486700
• NCBI GeneID: 7301
• NCBI Accession #: NP_001317193.1
• NCBI GenBank Nucleotide #: NM_001330264.1
• UniProt Accession #: Q06418; O14953; Q86VR3
• Molecular Weight: 96,905 Da
• NCBI Official Full Name: tyrosine-protein kinase receptor TYRO3 isoform 2
• NCBI Official Synonym Full Names: TYRO3 protein tyrosine kinase
• NCBI Official Symbol: TYRO3
• NCBI Official Synonym Symbols: BYK; Dtk; RSE; Rek; Sky; Tif; Etk-2
• NCBI Protein Information: tyrosine-protein kinase receptor TYRO3
• UniProt Protein Name: Tyrosine-protein kinase receptor TYRO3
• Protein Family: Tyrosine-protein kinase receptor
• UniProt Gene Name: TYRO3
• UniProt Synonym Gene Names: BYK; DTK; RSE; SKY; TIF
• UniProt Entry Name: TYRO3_HUMAN

NCBI Description

The gene is part of a 3-member transmembrane receptor kinase receptor family with a processed pseudogene distal on chromosome 15. The encoded protein is activated by the products of the growth arrest-specific gene 6 and protein S genes and is involved in controlling cell survival and proliferation, spermatogenesis, immunoregulation and phagocytosis. The encoded protein has also been identified as a cell entry factor for Ebola and Marburg viruses. [provided by RefSeq, May 2010]

Uniprot Description

Tyro3: Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of TYRO3 on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with PIK3R1 and thereby enhances PI3-kinase activity. Activates the AKT survival pathway, including nuclear translocation of NF-kappa-B and up-regulation of transcription of NF-kappa-B-regulated genes. TYRO3 signaling plays a role in various processes such as neuron protection from excitotoxic injury, platelet aggregation and cytoskeleton reorganization. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3. Monomer and homodimer. Interacts (via N-terminus) with extracellular ligands TUB, TULP1 and GAS6. Interacts with PIK3R1; this interaction increases PI3-kinase activity. Abundant in the brain and lower levels in other tissues. Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.

Protein type: Axl family; EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group

Chromosomal Location of Human Ortholog: 15q15

Cellular Component: endoplasmic reticulum membrane; nuclear envelope; nucleus

Molecular Function: phosphoinositide 3-kinase binding; protein binding; protein-tyrosine kinase activity

Biological Process: apoptotic cell clearance; forebrain cell migration; negative regulation of inflammatory response; negative regulation of innate immune response; negative regulation of neuron apoptosis; negative regulation of toll-like receptor signaling pathway; ovulation cycle; platelet activation; protein amino acid autophosphorylation; protein kinase B signaling cascade; secretion by cell; spermatogenesis