Human Heparin binding protein ELISA Kit | AZU/HBP elisa kit

Human Heparin binding protein ELISA Kit

Cat. Num. AAA739465

• Reactivity: Human
• Synonyms: Heparin binding protein; Human Heparin binding protein ELISA Kit; AZU/HBP elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive or Sandwich
• Detection Range: 5.0-100ng/mL
• Sensitivity: 1.0 ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AZU/HBP elisa kit

Intended Uses: This HBP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human HBP.

Principle of the Assay: HBP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for HBP. Standards or samples are then added to the microtiter plate wells and HBP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of HBP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for HBP are added to each well to "sandwich" the HBP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain HBP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HBP concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 183951
• Molecular Weight: 26,264 Da
• NCBI Official Full Name: heparin binding protein
• UniProt Protein Name: Fibroblast growth factor-binding protein 1
• UniProt Gene Name: FGFBP1
• UniProt Synonym Gene Names: FGFBP; HBP17; FGF-BP; FGF-BP1; FGF-binding protein 1; FGFBP-1; 17 kDa HBGF-binding protein; HBp17
• UniProt Entry Name: FGFP1_HUMAN

NCBI Description

This gene encodes a secreted fibroblast growth factor carrier protein. The encoded protein plays a critical role in cell proliferation, differentiation and migration by binding to fibroblast growth factors and potentiating their biological effects on target cells. The encoded protein may also play a role in tumor growth as an angiogenic switch molecule, and expression of this gene has been associated with several types of cancer including pancreatic and colorectal adenocarcinoma. A pseudogene of this gene is also located on the short arm of chromosome 4. [provided by RefSeq, Nov 2011]

Uniprot Description

FGFBP1: Acts as a carrier protein that release fibroblast- binding factors (FGFs) from the extracellular matrix (EM) storage and thus enhance the mitogenic activity of FGFs. Enhances FGF2 signaling during tissue repair, angiogenesis and in tumor growth. Belongs to the fibroblast growth factor-binding protein family.

Chromosomal Location of Human Ortholog: 4p15.32

Cellular Component: extracellular space; cell surface; plasma membrane

Molecular Function: heparin binding; protein binding; fibroblast growth factor binding

Biological Process: negative regulation of cell proliferation; cell-cell signaling; positive regulation of cell proliferation; positive regulation of fibroblast growth factor receptor signaling pathway; signal transduction