Goat Atrophin-1 Interacting Protein 4 Polyclonal Antibody | anti-ITCH antibody

Atrophin-1 Interacting Protein 4 (AIF4, ITCH, Itchy Homolog E3 Ubiquitin Protein Ligase (mouse), AIP4, NAPP1, dJ468O1.1, NFE2-associated Polypeptide 1, dJ468O1.1)

Cat. Num. AAA622191

• Gene Names: ITCH; AIF4; AIP4; NAPP1; dJ468O1.1
• Reactivity: Canine, Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Atrophin-1 Interacting Protein 4; Polyclonal Antibody; Atrophin-1 Interacting Protein 4 (AIF4; ITCH; Itchy Homolog E3 Ubiquitin Protein Ligase (mouse); AIP4; NAPP1; dJ468O1.1; NFE2-associated Polypeptide 1; dJ468O1.1); Anti -Atrophin-1 Interacting Protein 4 (AIF4; anti-ITCH antibody

• Host: Goat
• Reactivity: Canine, Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes human AIF4 at ~100kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 98.7kD according to NP_113671.3). Species Crossreactivity: Canine, mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris-saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-ITCH antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:32000; Western Blot: 0.03-0.1ug/ml
• Immunogen: Synthetic peptide of AIF4.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of AIF4 in Human Brain (Frontal Cortex) lysate (35μg protein in RIPA buffer) using AIF4 antibody (0.03μg/ml).)

Related Product Information for anti-ITCH antibody

Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral andpallidoluysian atrophy. The protein encoded by this gene interacts with atrophin-1. This encoded protein is a closely related member of the NEDD4-like protein family. This family of proteins are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. This encoded protein contains four tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. It can act as a transcriptional corepressor of p45/NFE2 and may participate in the regulation of immune responses by modifying Notch-mediated signaling. It is highly similar to the mouse Itch protein, which has been implicated in the regulation and differentiation of erythroid and lymphoid cells.

Product Categories/Family for anti-ITCH antibody

Antibodies; Abs to Ubiquitin Ligases

NCBI and Uniprot Product Information

• NCBI GI #: 2708329
• NCBI GeneID: 83737
• UniProt Accession #: Q59ER4; Q96J02
• Molecular Weight: 71,394 Da
• NCBI Official Full Name: atrophin-1 interacting protein 4
• NCBI Official Synonym Full Names: itchy E3 ubiquitin protein ligase homolog (mouse)
• NCBI Official Symbol: ITCH
• NCBI Official Synonym Symbols: AIF4; AIP4; NAPP1; dJ468O1.1
• NCBI Protein Information: E3 ubiquitin-protein ligase Itchy homolog; OTTHUMP00000030688; OTTHUMP00000030689; NFE2-associated polypeptide 1; ubiquitin protein ligase ITCH; atrophin-1 interacting protein 4; atrophin-1-interacting protein 4; itchy homolog E3 ubiquitin protein ligase; dJ468O1.1 (atrophin 1 interacting protein 4 (AIP4))
• UniProt Protein Name: Itchy homolog E3 ubiquitin protein ligase variant
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Entry Name: Q59ER4_HUMAN

NCBI Description

Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. The protein encoded by this gene interacts with atrophin-1. This encoded protein is a closely related member of the NEDD4-like protein family. This family of proteins are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. This encoded protein contains four tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. It can act as a transcriptional corepressor of p45/NFE2 and may participate in the regulation of immune responses by modifying Notch-mediated signaling. It is highly similar to the mouse Itch protein, which has been implicated in the regulation and differentiation of erythroid and lymphoid cells. [provided by RefSeq]

Uniprot Description

ITCH: an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It catalyzes 'Lys-29'-, 'Lys-48'- and 'Lys-63'-linked ubiquitin conjugation. It is involved in the control of inflammatory signaling pathways. Is an essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, TAX1BP1 and RNF11, that ensures the transient nature of inflammatory signaling pathways. Promotes the association of the complex after TNF stimulation. Once the complex is formed, TNFAIP3 deubiquitinates 'Lys-63' polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NFKB1. Ubiquitinates RIPK2 by 'Lys-63'-linked conjugation and influences NOD2-dependent signal transduction pathways. Regulates the transcriptional activity of several transcription factors, and probably plays an important role in the regulation of immune response. Ubiquitinates NFE2 by 'Lys-63' linkages and is implicated in the control of the development of hematopoietic lineages. Critical regulator of T-helper (TH2) cytokine development through its ability to induce JUNB ubiquitination and degradation. Ubiquitinates SNX9. Ubiquitinates CXCR4 and HGS/HRS and regulates sorting of CXCR4 to the degradative pathway. It is involved in the negative regulation of MAVS-dependent cellular antiviral responses. Ubiquitinates MAVS through 'Lys-48'-linked conjugation resulting in MAVS proteasomal degradation. Involved in the regulation of apoptosis and reactive oxygen species levels through the ubiquitination and proteasomal degradation of TXNIP. Mediates the antiapoptotic activity of epidermal growth factor through the ubiquitination and proteasomal degradation of p15 BID. Targets DTX1 for lysosomal degradation and controls NOTCH1 degradation, in the absence of ligand, through 'Lys-29'-linked polyubiquitination. Monomer Interacts (via its WW domains) with OCNL, NOTCH1 AND JUN. Interacts with JUNB; the interaction promotes ITCH-mediated ubiquitination and degradation of JUNB. Interacts with NDFIP1 and NDFIP2; this interaction activates the E3 ubiquitin-protein ligase and may induce its recruitment to exosomes. Interacts with ARHGEF7. Interacts with RNF11. Interacts (via the WW 1 domain) with NFE2 (via the PXY motif 1); the interaction promotes 'Lys-63'-linked ubiquitination of NFE2, retains it in the cytoplasm and prevents its transactivation activity. Interacts with FYN; the interaction phosphorylates ITCH on Tyr-420 decreasing binding of JUNB. Interacts (via WW domains) with CXCR4 (via C-terminus); the interaction depends on CXCR4 phosphorylation. Interacts (via WW domains) with PCBP2 within a complex containing ITCH, MAVS and PCBP2. Interacts (via WW domains) with TXNIP (via C-terminus). Interacts with ERBB4, DTX1, SPG20, SNX9 and SNX18. Interacts (via its WW domains) with ATN1. Interacts with Epstein-Barr virus LMP2A. Interacts (via WW domains) with SGK3. Widely expressed. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; EC 6.3.2.-; EC 6.3.2.19; Ligase; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 20q11.22

Cellular Component: membrane; cytoplasm; plasma membrane; cell cortex; cytoplasmic vesicle; cytosol; nucleus

Molecular Function: ribonucleoprotein binding; protein binding; CXCR chemokine receptor binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; negative regulation of JNK cascade; Notch signaling pathway; entry of virus into host cell; apoptosis; protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination; negative regulation of defense response to virus; inhibition of NF-kappaB transcription factor; positive regulation of protein catabolic process; innate immune response; negative regulation of alpha-beta T cell proliferation; regulation of cell growth; inflammatory response; negative regulation of interferon type I production; defense response to virus; positive regulation of T cell anergy; negative regulation of apoptosis

Disease: Autoimmune Disease, Multisystem, With Facial Dysmorphism