Bovine Ataxin 1 ELISA Kit | ATXN1 elisa kit

Bovine Ataxin 1 ELISA Kit

Cat. Num. AAA742749

• Gene Names: ATXN1; ATX1; SCA1; D6S504E
• Reactivity: Bovine
• Synonyms: Ataxin 1; Bovine Ataxin 1 ELISA Kit; ATXN1 elisa kit

• Reactivity: Bovine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Intended Uses: This ATXN1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine ATXN1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ATXN1 elisa kit

Principle of the Assay: ATXN1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ATXN1 antibody and an ATXN1-HRP conjugate. The assay sample and buffer are incubated together with ATXN1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATXN1 concentration since ATXN1 from samples and ATXN1-HRP conjugate compete for the anti-ATXN1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ATXN1 from the sample, fewer sites are left to bind ATXN1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATXN1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ATXN1 elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 189491748
• NCBI GeneID: 6310
• NCBI Accession #: NP_001121636.1
• NCBI GenBank Nucleotide #: NM_001128164.1
• Molecular Weight: 86,923 Da
• NCBI Official Full Name: ataxin-1
• NCBI Official Synonym Full Names: ataxin 1
• NCBI Official Symbol: ATXN1
• NCBI Official Synonym Symbols: ATX1; SCA1; D6S504E
• NCBI Protein Information: ataxin-1; alternative ataxin1; spinocerebellar ataxia type 1 protein
• UniProt Protein Name: Ataxin-1
• Protein Family: Ataxin
• UniProt Gene Name: ATXN1
• UniProt Synonym Gene Names: ATX1; SCA1
• UniProt Entry Name: ATX1_HUMAN

NCBI Description

The autosomal dominant cerebellar ataxias (ADCA) are a heterogeneous group of neurodegenerative disorders characterized by progressive degeneration of the cerebellum, brain stem and spinal cord. Clinically, ADCA has been divided into three groups: ADCA types I-III. ADCAI is genetically heterogeneous, with five genetic loci, designated spinocerebellar ataxia (SCA) 1, 2, 3, 4 and 6, being assigned to five different chromosomes. ADCAII, which always presents with retinal degeneration (SCA7), and ADCAIII often referred to as the `pure' cerebellar syndrome (SCA5), are most likely homogeneous disorders. Several SCA genes have been cloned and shown to contain CAG repeats in their coding regions. ADCA is caused by the expansion of the CAG repeats, producing an elongated polyglutamine tract in the corresponding protein. The expanded repeats are variable in size and unstable, usually increasing in size when transmitted to successive generations. The function of the ataxins is not known. This locus has been mapped to chromosome 6, and it has been determined that the diseased allele contains 41-81 CAG repeats, compared to 6-39 in the normal allele, and is associated with spinocerebellar ataxia type 1 (SCA1). At least two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jan 2010]

Uniprot Description

ataxin-1: spinocerebellar ataxia type 1 (SCA1) protein. Expansion of CAG repeats in the SCA1 gene results in an abnormally long polyglutamine tract in the protein, causing polyglutamine-induced neurodegeneration. 14-3-3 and Akt kinase stabilize both wild-type and mutant forms of ataxin-1. The expansion of the polyglutamine tract may alter this function.

Protein type: RNA-binding; Nuclear export

Chromosomal Location of Human Ortholog: 6p23

Cellular Component: nucleoplasm; nuclear RNA export factor complex; nuclear matrix; intracellular membrane-bound organelle; cytoplasm; nucleus; nuclear inclusion body

Molecular Function: protein C-terminus binding; identical protein binding; protein binding; protein self-association; DNA binding; poly(U) binding; chromatin binding; poly(rG) binding

Biological Process: RNA processing; transcription, DNA-dependent; adult locomotory behavior; nuclear export; negative regulation of insulin-like growth factor receptor signaling pathway; negative regulation of phosphorylation; visual learning; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; regulation of excitatory postsynaptic membrane potential; alveolus development

Disease: Spinocerebellar Ataxia 1