Rabbit anti-Mouse Prkcg Polyclonal Antibody | anti-Prkcg antibody

Prkcg, NT (Prkcg, Pkcc, Pkcg, Prkcc, Protein kinase C gamma type) (PE)

Cat. Num. AAA6337357

• Gene Names: Prkcg; Pkcc; Prkcc; PKCgamma
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Prkcg; Polyclonal Antibody; NT (Prkcg; Pkcc; Pkcg; Prkcc; Protein kinase C gamma type) (PE); Protein kinase C gamma type; anti-Prkcg antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-Prkcg antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Prkcg antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 73-102 amino acids from the N-terminal region of Mouse Prkcg.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Mouse Prkcg Antibody (N-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the Prkcg antibody detected the Prkcg protein (arrow).)

Related Product Information for anti-Prkcg antibody

This is a calcium-activated, phospholipid-dependent, serine-and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

Product Categories/Family for anti-Prkcg antibody

Antibodies; Protein Kinases

References

Seki, T., et al. J. Biol. Chem. 285(43):33252-33264(2010). Deng, P.Y., et al. J. Neurosci. 30(15):5136-5148(2010). Ross, S.E., et al. Neuron 65(6):886-898(2010). Brzezinski, J.A. IV, et al. Development 137(4):619-629(2010). Tao, H., et al. Proc. Natl. Acad. Sci. U.S.A. 106(34):14426-14431(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 6755080
• NCBI GeneID: 18752
• NCBI Accession #: NP_035232.1
• NCBI GenBank Nucleotide #: NM_011102.4
• UniProt Accession #: P63318; P05697
• Molecular Weight: 78,358 Da
• NCBI Official Full Name: protein kinase C gamma type isoform 1
• NCBI Official Synonym Full Names: protein kinase C, gamma
• NCBI Official Symbol: Prkcg
• NCBI Official Synonym Symbols: Pkcc; Prkcc; PKCgamma
• NCBI Protein Information: protein kinase C gamma type
• UniProt Protein Name: Protein kinase C gamma type
• Protein Family: Protein kinase
• UniProt Gene Name: Prkcg
• UniProt Synonym Gene Names: Pkcc; Pkcg; Prkcc; PKC-gamma
• UniProt Entry Name: KPCG_MOUSE

Uniprot Description

PKCG: a calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase. Expressed in the brain and spinal cord where its localization is restricted to neurons. Several neuronal functions, including long term potentiation and depression (LTP&LTD) specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects have been associated with neurodegenerative disorder spinocerebellar ataxia-14. Plays diverse roles in neuronal cells and eye tissues, such as regulation of the neuronal receptors GLUR4 and NMDAR1, modulation of receptors and neuronal functions related to sensitivity to opiates, pain and alcohol, mediation of synaptic function and cell survival after ischemia, and inhibition of gap junction activity after oxidative stress. Binds and phosphorylates GLUR4 glutamate receptor and regulates its function by increasing plasma membrane-associated GRIA4 expression. In primary cerebellar neurons treated with the agonist 3,5-dihyidroxyphenylglycine, functions downstream of the metabotropic glutamate receptor MGLUR5 and phosphorylates NMDAR1 receptor which plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. May be involved in the regulation of hippocampal long-term potentiation (LTP), but may be not necessary for the process of synaptic plasticity. May modulate the functionality of mu-type-opioid receptors by participating in a signaling pathway which leads to the phosphorylation and degradation of opioid receptors. May also contributes to chronic morphine-induced changes in nociceptive processing. Plays a role in neuropathic pain mechanisms and contributes to the maintenance of the allodynia pain produced by peripheral inflammation. Plays an important role in initial sensitivity and tolerance to ethanol, by mediating the behavioral effects of ethanol as well as the effects of this drug on the GABA(A) receptors. During and after cerebral ischemia modulate neurotransmission and cell survival in synaptic membranes, and is involved in insulin-induced inhibition of necrosis, an important mechanism for minimizing ischemic injury. Required for the elimination of multiple climbing fibers during innervation of Purkinje cells in developing cerebellum. Is activated in lens epithelial cells upon hydrogen peroxide treatment, and phosphorylates connexin-43, resulting in disassembly of GJA1 gap junction plaques and inhibition of gap junction activity which could provide a protective effect against oxidative stress. Phosphorylates p53 and promotes p53-dependent apoptosis in response to DNA damage. Interacts with GRIA4. Interacts with CDCP1. Interacts with TP53INP1 and p53. Expressed in Purkinje cells of the cerebellar cortex.

Protein type: EC 2.7.11.13; Kinase, protein; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); AGC group; PKC family; Alpha subfamily

Cellular Component: cell junction; cell projection; cytoplasm; cytosol; dendrite; intercellular junction; membrane; neuron projection; nucleus; perinuclear region of cytoplasm; plasma membrane; synapse

Molecular Function: ATP binding; calcium-dependent protein kinase C activity; kinase activity; metal ion binding; nucleotide binding; protein binding; protein kinase activity; protein kinase C activity; protein serine/threonine kinase activity; protein serine/threonine/tyrosine kinase activity; transferase activity; zinc ion binding

Biological Process: chemosensory behavior; negative regulation of neuron apoptosis; negative regulation of protein catabolic process; negative regulation of protein ubiquitination; peptidyl-serine phosphorylation; phosphorylation; positive regulation of mismatch repair; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of circadian rhythm; regulation of phagocytosis; regulation of response to food; response to morphine; response to pain; rhythmic process; synaptic transmission