Rabbit anti-Human ATPIF1 Polyclonal Antibody | anti-ATPIF1 antibody

ATPIF1 Antibody

Cat. Num. AAA9408958

• Gene Names: ATPIF1; IP; ATPI; ATPIP
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Antigen affinity purification.
• Synonyms: ATPIF1; Polyclonal Antibody; ATPIF1 Antibody; IP; ATPI; ATPIP; anti-ATPIF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total ATPIF1 protein.
• Purity/Purification: Antigen affinity purification.
• Form/Format: Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
• Concentration: 2.1 mg/ml (varies by lot)
• Sequence Length: 106

• Applicable Applications for anti-ATPIF1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:1000-1:5000; Immunohistochemistry: 1:50-1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Fusion protein corresponding to a region derived from internal residues of human ATPase inhibitory factor 1
• Target Name: ATPIF1
• Preparation and Storage: Store at -20 degree C

Testing Data

(Gel: 16%+12%+10%SDS-PAGE Lysates (from left to right): Hela, Jurkat, MCF7 and A431 cell Amount of lysate: 40ug per lane Primary antibody: 1/1200 dilution Secondary antibody dilution: 1/8000 Exposure time: 2 minutes)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using at dilution 1/50.)

Related Product Information for anti-ATPIF1 antibody

ATPase inhibitor, mitochondrial is an enzyme that in humans is encoded by the ATPIF1 gene. This gene encodes a mitochondrial ATPase inhibitor. Alternative splicing occurs at this locus and three transcript variants encoding distinct isoforms have been identified. It prevents ATPase from switching to ATP hydrolysis during collapse of the electrochemical gradient, for example during oxygen deprivation ATP synthase inhibitor forms a one to one complex with the F1 ATPase, possibly by binding at the alpha-beta interface. It is thought to inhibit ATP synthesis by preventing the release of ATP. The inhibitor has two oligomeric states, dimer (the active state) and tetramer.

Product Categories/Family for anti-ATPIF1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 7705927
• NCBI GeneID: 93974
• NCBI Accession #: NP_057395.1
• NCBI GenBank Nucleotide #: NM_016311.4
• UniProt Accession #: Q9UII2; Q5JXL8; Q6IAQ7; Q9BSL9
• Molecular Weight: 6,592 Da
• NCBI Official Full Name: ATPase inhibitor, mitochondrial isoform 1
• NCBI Official Synonym Full Names: ATPase inhibitory factor 1
• NCBI Official Symbol: ATPIF1
• NCBI Official Synonym Symbols: IP; ATPI; ATPIP
• NCBI Protein Information: ATPase inhibitor, mitochondrial
• UniProt Protein Name: ATPase inhibitor, mitochondrial
• Protein Family: ATPase inhibitor
• UniProt Gene Name: ATPIF1
• UniProt Synonym Gene Names: ATPI; IF(1); IF1
• UniProt Entry Name: ATIF1_HUMAN

NCBI Description

This gene encodes a mitochondrial ATPase inhibitor. Alternative splicing occurs at this locus and three transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

ATPIF1: Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. Homodimer at a pH value below 6.5, inactive homotetramer above. Belongs to the ATPase inhibitor family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Inhibitor

Chromosomal Location of Human Ortholog: 1p35.3

Cellular Component: cell surface; mitochondrion; mitochondrial proton-transporting ATP synthase complex

Molecular Function: enzyme inhibitor activity; calmodulin binding; angiostatin binding; protein homodimerization activity; ATPase inhibitor activity; ATPase binding

Biological Process: generation of precursor metabolites and energy; regulation of gene expression; negative regulation of hydrolase activity; negative regulation of endothelial cell proliferation; erythrocyte differentiation; angiogenesis; mitochondrial depolarization; protein homooligomerization; negative regulation of nucleotide metabolic process; heme biosynthetic process; protein homotetramerization