Western Blot (WB)
(Western Blot detection against Immunogen (36.63kD).)
Mouse anti-Human, Mouse ATG5 Monoclonal Antibody | anti-ATG5 antibody
ATG5 (Autophagy Protein 5, APG5-like, Apoptosis-specific Protein, APG5L, ASP) (AP)
Gene Names
ATG5; ASP; APG5; APG5L; hAPG5; APG5-LIKE
Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
ATG5; Monoclonal Antibody; ATG5 (Autophagy Protein 5; APG5-like; Apoptosis-specific Protein; APG5L; ASP) (AP); anti-ATG5 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
3D2
Specificity
Recognizes human APG5L.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-ATG5 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Partial recombinant corresponding to aa176-275 from APG5L (AAH02699) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
PAEENGFRYIPFRIYQTTTERPFIQKLFRPVAADGQLHTLGDLLKEVCPSAIDPEDGEKKNQVMIHGIEPMLETPLQWLSEHLSYPDNFLHISIIPQPTD
Conjugate
AP
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western Blot detection against Immunogen (36.63kD).)
Western Blot (WB)
(Western Blot detection against Immunogen (36.63kD).)
Related Product Information for anti-ATG5 antibody
ATG5 is required for the process of autophagy, an intracellular process for bulk degradation of cytoplasmic components. ATG5 conjugates to ATG12 and associates with isolation membranes to form autophagosomes. The autophagosomes then enclose cytoplasmic components and deliver them to lysosomes/vacuoles to be degraded. ATG5 has been shown to be involved in apoptosis with expression late in the apoptotic process downstream of caspase activity.
Product Categories/Family for anti-ATG5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
22,931 Da
NCBI Official Full Name
Homo sapiens ATG5 autophagy related 5 homolog (S. cerevisiae), mRNA
NCBI Official Synonym Full Names
autophagy related 5
NCBI Official Symbol
ATG5
NCBI Official Synonym Symbols
ASP; APG5; APG5L; hAPG5; APG5-LIKE
NCBI Protein Information
autophagy protein 5
Protein Family
NCBI Description
The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
Research Articles on ATG5
Similar Products
Product Notes
The ATG5 (Catalog #AAA6130115) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ATG5 (Autophagy Protein 5, APG5-like, Apoptosis-specific Protein, APG5L, ASP) (AP) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ATG5 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ATG5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATG5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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