Human Angiotensin II Receptor 2 ELISA Kit | AGTR2 elisa kit

Human Angiotensin II Receptor 2 ELISA Kit

Cat. Num. AAA731473

• Gene Names: AGTR2; AT2; ATGR2; MRX88
• Reactivity: Human
• Synonyms: Angiotensin II Receptor 2; Human Angiotensin II Receptor 2 ELISA Kit; AGTR2 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This AGTR2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human AGTR2. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AGTR2 elisa kit

Principle of the Assay: AGTR2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AGTR2 antibody and an AGTR2-HRP conjugate. The assay sample and buffer are incubated together with AGTR2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AGTR2 concentration since AGTR2 from samples and AGTR2-HRP conjugate compete for the anti-AGTR2 antibody binding site. Since the number of sites is limited, as more sites are occupied by AGTR2 from the sample, fewer sites are left to bind AGTR2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AGTR2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for AGTR2 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 49456893
• NCBI GeneID: 186
• UniProt Accession #: P50052; Q13016; Q6FGY7; B2R9V1; AAB34100
• Molecular Weight: 41,184 Da
• NCBI Official Full Name: AGTR2, partial
• NCBI Official Synonym Full Names: angiotensin II receptor, type 2
• NCBI Official Symbol: AGTR2
• NCBI Official Synonym Symbols: AT2; ATGR2; MRX88
• NCBI Protein Information: type-2 angiotensin II receptor; angiotensin II type-2 receptor
• UniProt Protein Name: Type-2 angiotensin II receptor
• Protein Family: Type-2 angiotensin II receptor
• UniProt Gene Name: AGTR2
• UniProt Synonym Gene Names: AT2
• UniProt Entry Name: AGTR2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the G-protein coupled receptor 1 family, and functions as a receptor for angiotensin II. It is an intergral membrane protein that is highly expressed in fetus, but scantily in adult tissues, except brain, adrenal medulla, and atretic ovary. This receptor has been shown to mediate programmed cell death and this apoptotic function may play an important role in developmental biology and pathophysiology. Mutations in this gene are been associated with X-linked mental retardation. [provided by RefSeq, Jan 2010]

Uniprot Description

AT2: Receptor for angiotensin II. Cooperates with MTUS1 to inhibit ERK2 activation and cell proliferation. Defects in AGTR2 are the cause of mental retardation X- linked type 88 (MRX88). Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Belongs to the G-protein coupled receptor 1 family.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: Xq22-q23

Cellular Component: perinuclear region of cytoplasm; integral to plasma membrane; plasma membrane; extracellular region

Molecular Function: receptor antagonist activity; protein binding; angiotensin type II receptor activity; peptide hormone binding; transcription factor binding

Biological Process: positive regulation of phosphoprotein phosphatase activity; regulation of transcription factor import into nucleus; negative regulation of icosanoid secretion; negative regulation of heart rate; positive regulation of nitric oxide biosynthetic process; negative regulation of nerve growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; renin-angiotensin regulation of aldosterone production; dopamine biosynthetic process; positive regulation of vasodilation; angiotensin mediated vasodilation involved in regulation of systemic arterial blood pressure; cerebellar cortex development; regulation of systemic arterial blood pressure by circulatory renin-angiotensin; cell surface receptor linked signal transduction; regulation of blood pressure; positive regulation of cell proliferation; inflammatory response; cellular sodium ion homeostasis; positive regulation of nitric-oxide synthase activity; negative regulation of blood vessel endothelial cell migration; response to organic nitrogen; brain renin-angiotensin system; G-protein coupled receptor protein signaling pathway; negative regulation of fibroblast proliferation; blood vessel remodeling; G-protein signaling, coupled to cGMP nucleotide second messenger; brain development; negative regulation of cell growth; nitric oxide mediated signal transduction; positive regulation of cytokine secretion