Typical Testing Data/Standard Curve (for reference only)
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Superoxide Anion Assay Kit
Superoxide Anion Colorimetric Assay Kit
Synonyms
Superoxide Anion; Superoxide Anion Colorimetric Assay Kit; Superoxide Anion assay kit
Application Notes
The kit provides a simple method for detecting the O2- content in a variety of biological samples such as animal tissues, plant tissues, serum, cells. In the assay, The superoxide anion in variety of biological samples reacts with hydroxylamine hydrochloride to produce NO2-, and NO2- reacts with Gris' reagent. The mechanism of Gris' analysis is summarized as the azo coupling between diazoniums, which is It is produced by sulfonamides and NO2- and N-(1-naphthyl)ethylenediamine dihydrochloride to generate a red azo compound with a characteristic absorption peak at 540nm. The O2- content in the sample can be calculated based on the A540 value.
Preparation and Storage
Store at 4 degree C and keep from light immediately upon receipt. Kit has a storage time of est. 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Shipping: Gel pack with blue ice.
Shipping: Gel pack with blue ice.
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Related Product Information for Superoxide Anion assay kit
Principle of the Assay: The superoxide anion in plant, animal tissue, serum and other samples reacts with hydroxylamine hydrochloride to produce NO2?, and NO2? reacts with Gris' reagent. The mechanism of Gris analysis is summarized as the azo coupling between diazoniums, which is It is produced by sulfonamides and NO2? and N-(1-naphthyl)ethylenediamine dihydrochloride to generate a red azo compound with a characteristic absorption peak at 540nm. The O2- content in the sample can be calculated based on the A540 value. The kit can detect samples of plants, animal tissues, serum and cells.
Background/Introduction: Reactive oxygen species such as superoxide anions in organisms have immune and signal transduction effects, but when they accumulate too much, they will destroy cell membranes and biological macromolecules, leading to abnormal metabolism of cells and tissues in the body, causing many diseases.
Background/Introduction: Reactive oxygen species such as superoxide anions in organisms have immune and signal transduction effects, but when they accumulate too much, they will destroy cell membranes and biological macromolecules, leading to abnormal metabolism of cells and tissues in the body, causing many diseases.
Product Categories/Family for Superoxide Anion assay kit
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Product Notes
The Superoxide Anion (Catalog #AAA9719091) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The kit provides a simple method for detecting the O2- content in a variety of biological samples such as animal tissues, plant tissues, serum, cells. In the assay, The superoxide anion in variety of biological samples reacts with hydroxylamine hydrochloride to produce NO2-, and NO2- reacts with Gris' reagent. The mechanism of Gris' analysis is summarized as the azo coupling between diazoniums, which is It is produced by sulfonamides and NO2- and N-(1-naphthyl)ethylenediamine dihydrochloride to generate a red azo compound with a characteristic absorption peak at 540nm. The O2- content in the sample can be calculated based on the A540 value. Researchers should empirically determine the suitability of the Superoxide Anion for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Superoxide Anion, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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