Western Blot (WB)
(NT5C1B Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the NT5C1B antibody detected the NT5C1B protein (arrow).)
Rabbit anti-Human, Mouse NT5C1B Polyclonal Antibody | anti-NT5C1B antibody
NT5C1B, NT (NT5C1B, AIRP, Cytosolic 5'-nucleotidase 1B, Autoimmune infertility-related protein, Cytosolic 5'-nucleotidase IB) (Azide free) (HRP)
Gene Names
NT5C1B; AIRP; CN1B; CN-IB
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
NT5C1B; Polyclonal Antibody; NT (NT5C1B; AIRP; Cytosolic 5'-nucleotidase 1B; Autoimmune infertility-related protein; Cytosolic 5'-nucleotidase IB) (Azide free) (HRP); Cytosolic 5'-nucleotidase IB; anti-NT5C1B antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-NT5C1B antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
NT5C1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10-40 amino acids from the N-terminal region of human NT5C1B.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(NT5C1B Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the NT5C1B antibody detected the NT5C1B protein (arrow).)
Western Blot (WB)
(NT5C1B Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the NT5C1B antibody detected the NT5C1B protein (arrow).)
Related Product Information for anti-NT5C1B antibody
Cytosolic 5-prime nucleotidases, such as NT5C1B, catalyze production of adenosine, which regulates diverse physiologic processes (Sala-Newby and Newby, 2001 [PubMed 11690631]).[supplied by OMIM].
Product Categories/Family for anti-NT5C1B antibody
References
Gass, N., et al. J Affect Disord 126 (1-2), 134-139 (2010): Cirulli, E.T., et al. Eur. J. Hum. Genet. 18(7):815-820(2010). Olsen, J.V., et al. Cell 127(3):635-648(2006). Sala-Newby, G.B., et al. Biochim. Biophys. Acta 1521 (1-3), 12-18 (2001).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
68,804 Da
NCBI Official Full Name
cytosolic 5'-nucleotidase 1B isoform 1
NCBI Official Synonym Full Names
5'-nucleotidase, cytosolic IB
NCBI Official Symbol
NT5C1B
NCBI Official Synonym Symbols
AIRP; CN1B; CN-IB
NCBI Protein Information
cytosolic 5'-nucleotidase 1B; autoimmune infertility-related protein
UniProt Protein Name
Cytosolic 5'-nucleotidase 1B
Protein Family
UniProt Gene Name
NT5C1B
UniProt Synonym Gene Names
AIRP; cN1B; cN-IB
UniProt Entry Name
5NT1B_HUMAN
NCBI Description
Cytosolic 5-prime nucleotidases, such as NT5C1B, catalyze production of adenosine, which regulates diverse physiologic processes (Sala-Newby and Newby, 2001 [PubMed 11690631]).[supplied by OMIM, Mar 2008]
Uniprot Description
NT5C1B: Dephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides. Helps to regulate adenosine levels. Belongs to the 5'-nucleotidase type 3 family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Nucleotide Metabolism - pyrimidine; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; EC 3.1.3.5; Nucleotide Metabolism - purine; Hydrolase
Chromosomal Location of Human Ortholog: 2p24.2
Cellular Component: cytosol; nucleus
Molecular Function: 5'-nucleotidase activity; magnesium ion binding; nucleotide binding
Biological Process: dephosphorylation; nucleobase, nucleoside and nucleotide metabolic process; purine nucleotide catabolic process; purine base metabolic process
Research Articles on NT5C1B
Similar Products
Product Notes
The NT5C1B nt5c1b (Catalog #AAA6326923) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NT5C1B, NT (NT5C1B, AIRP, Cytosolic 5'-nucleotidase 1B, Autoimmune infertility-related protein, Cytosolic 5'-nucleotidase IB) (Azide free) (HRP) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NT5C1B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the NT5C1B nt5c1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NT5C1B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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