Rabbit anti-Human ATF4 Polyclonal Antibody | anti-ATF4 antibody

ATF4 (S245) (Activating Transcription Factor 4 (Tax-responsive Enhancer Element B67), Activating Transcription Factor 4 (Tax-responsive Enhancer Element B67), Isoform CRA_a,, ATF4 Protein, hCG_41721) (AP)

Cat. Num. AAA6461279

• Gene Names: ATF4; CREB2; TXREB; CREB-2; TAXREB67
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: ATF4; Polyclonal Antibody; ATF4 (S245) (Activating Transcription Factor 4 (Tax-responsive Enhancer Element B67); Activating Transcription Factor 4 (Tax-responsive Enhancer Element B67); Isoform CRA_a; ; ATF4 Protein; hCG_41721) (AP); anti-ATF4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ATF4.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-ATF4 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to amino acid residues surrounding S245 of human ATF4 (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ATF4 (arrow) using rabbit anti-ATF4 Antibody (S245). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ATF4 gene (Lane 2) (Origene Technologies).)

Western Blot (WB)

(Western blot analysis of ATF4 Antibody (S245) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in WiDr cell line lysate. ATF4 Antibody (pS245) (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-ATF4 antibody

Antibodies; Transcription Factors

References

1. Gombart, A.F., J. Leukoc. Biol. 81(6), 1535-1547 (2007). 2. Jousse, C., J. Biol. Chem. 282(21), 15851-15861 (2007). 3. Kakiuchi, C., Neurosci. Lett. 417(3), 316-321 (2007). 4. Marchand, A., J. Biol. Chem. 281(28), 19124-19133 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 33469976
• NCBI GeneID: 468
• NCBI Accession #: NP_001666
• NCBI GenBank Nucleotide #: NM_001675.4
• UniProt Accession #: P18848; Q96AQ3
• Molecular Weight: 56.6 kDa (510aa)
• NCBI Official Full Name: cyclic AMP-dependent transcription factor ATF-4
• NCBI Official Synonym Full Names: activating transcription factor 4
• NCBI Official Symbol: ATF4
• NCBI Official Synonym Symbols: CREB2; TXREB; CREB-2; TAXREB67
• NCBI Protein Information: cyclic AMP-dependent transcription factor ATF-4
• UniProt Protein Name: Cyclic AMP-dependent transcription factor ATF-4
• Protein Family: Cyclic AMP-dependent transcription factor
• UniProt Gene Name: ATF4
• UniProt Synonym Gene Names: CREB2; TXREB; cAMP-dependent transcription factor ATF-4; CREB-2; cAMP-responsive element-binding protein 2; TaxREB67
• UniProt Entry Name: ATF4_HUMAN

NCBI Description

This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication. [provided by RefSeq, Sep 2011]

Uniprot Description

ATF-4: a transcription factor that is a member of the leucine zipper family. Isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). Involved in cisplatin resistance. Other members of the family include AP-1 transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. Two alternatively spliced isoforms have been described.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: nucleoplasm; neuron projection; cytoplasm; microtubule organizing center; nucleus

Molecular Function: protein C-terminus binding; protein binding; leucine zipper domain binding; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; amino acid metabolic process; unfolded protein response; positive regulation of apoptosis; negative regulation of potassium ion transport; positive regulation of transcription, DNA-dependent; gluconeogenesis; positive regulation of transcription from RNA polymerase I promoter; cellular response to glucose starvation; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; regulation of transcription, DNA-dependent; positive regulation of neuron apoptosis; positive regulation of transcription from RNA polymerase II promoter; circadian regulation of gene expression; gamma-aminobutyric acid signaling pathway