Testing Data
(Active chymotrypsin-like serine protease activity staining by flow cytometry. Jurkat cells were treated as shown with 1mM staurosporine to induce apoptosis. The cells were then stained using the FLISP FAM-Phe-DAP Serine Protease Assay Kit including propidium iodide . Apoptotic cells positive for serine protease activity can be seen in the bottom right quadrant and dead cells positive for both serine protease and PI in the top right quadrant. Healthy cells are negative for both stains. Data acquired on the ZE5 Cell Analyzer.)
Serine Protease Assay Kit
FLISP FAM-Leu-CMK Serine Protease Assay Kit
Applications
Flow Cytometry, Functional Assay, Immunofluorescence
Synonyms
Serine Protease; FLISP FAM-Leu-CMK Serine Protease Assay Kit; Serine Protease Assay Kit; Serine Protease assay kit
Applicable Applications for Serine Protease assay kit
Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Conjugation
FAM (Green Fluorescence)
Preparation and Storage
Store the unopened kit (and each unopened component) according to the storage instructions on each component label.
Store the FLISP reagent at -20 degree C. Once reconstituted in DMSO, the FLISP stock should be stored at -20 degree C for 6 months, protected from light.
Avoid repeated freezing and thawing.
Store the FLISP reagent at -20 degree C. Once reconstituted in DMSO, the FLISP stock should be stored at -20 degree C for 6 months, protected from light.
Avoid repeated freezing and thawing.
Testing Data
(Active chymotrypsin-like serine protease activity staining by flow cytometry. Jurkat cells were treated as shown with 1mM staurosporine to induce apoptosis. The cells were then stained using the FLISP FAM-Phe-DAP Serine Protease Assay Kit including propidium iodide . Apoptotic cells positive for serine protease activity can be seen in the bottom right quadrant and dead cells positive for both serine protease and PI in the top right quadrant. Healthy cells are negative for both stains. Data acquired on the ZE5 Cell Analyzer.)
Testing Data
(Active chymotrypsin-like serine protease activity staining by flow cytometry. Jurkat cells were treated as shown with 1mM staurosporine to induce apoptosis. The cells were then stained using the FLISP FAM-Phe-DAP Serine Protease Assay Kit including propidium iodide . Apoptotic cells positive for serine protease activity can be seen in the bottom right quadrant and dead cells positive for both serine protease and PI in the top right quadrant. Healthy cells are negative for both stains. Data acquired on the ZE5 Cell Analyzer.)
Related Product Information for Serine Protease assay kit
Summary: Fluorescent Labeled Inhibitors of Serine Protease (FLISP) kits allow the analysis of intracellular chymotrypsin-like serine protease activity in live cells. Serine protease activation plays major roles in several different cellular functions including apoptosis, tumor malignancy and as prognostic indicators of breast, head and neck carcinomas. Serine proteases are also thought to play a role in infections and transplant rejection.
Test Principle: Active chymotrypsin-like enzymes within the cell bind covalently to the FLISP FAM-Leu-CMK inhibitor probe, retaining the green fluorescent signal within the cell. The higher the concentration of active chymotrypsin-like enzymes within the cell, the higher the level of fluorescence observed. FLISP kits are suitable for use with suspension or adherent cells and can be used with a flow cytometer, fluorescence microscope or a fluorescence plate reader.
FLISP inhibitors are cell-permeable and non-cytotoxic fluorochrome-labeled analogs of the first serine protease inhibitor, tosyl-phenlalanylchloromethyl ketone (TPCK), labeled with carboxyfluorescein (FAM) which fluoresces green. They are available with either a chloromethyl ketone (CMK) or diphenyl 1-(N-peptidylamino) alkanephosphonate (DAP) reactive group containing compound. Specificity for chymotrypsin-like enzymes is determined by the amino acid targeting peptide, the FAM-Leu-CMK probe inhibits chymotrypsin C.
Test Principle: Active chymotrypsin-like enzymes within the cell bind covalently to the FLISP FAM-Leu-CMK inhibitor probe, retaining the green fluorescent signal within the cell. The higher the concentration of active chymotrypsin-like enzymes within the cell, the higher the level of fluorescence observed. FLISP kits are suitable for use with suspension or adherent cells and can be used with a flow cytometer, fluorescence microscope or a fluorescence plate reader.
FLISP inhibitors are cell-permeable and non-cytotoxic fluorochrome-labeled analogs of the first serine protease inhibitor, tosyl-phenlalanylchloromethyl ketone (TPCK), labeled with carboxyfluorescein (FAM) which fluoresces green. They are available with either a chloromethyl ketone (CMK) or diphenyl 1-(N-peptidylamino) alkanephosphonate (DAP) reactive group containing compound. Specificity for chymotrypsin-like enzymes is determined by the amino acid targeting peptide, the FAM-Leu-CMK probe inhibits chymotrypsin C.
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Product Notes
The Serine Protease (Catalog #AAA225615) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Serine Protease can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF). Researchers should empirically determine the suitability of the Serine Protease for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Serine Protease, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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