Ribulose Bisphosphate Carboxylase/Oxygenase (Rubisco) Assay Kit
Ribulose Bisphosphate Carboxylase/Oxygenase (Rubisco) Colorimetric Assay Kit
Synonyms
Ribulose Bisphosphate Carboxylase/Oxygenase (Rubisco); Ribulose Bisphosphate Carboxylase/Oxygenase (Rubisco) Colorimetric Assay Kit; Rubisco; Bisphosphate; Carboxylase/Oxygenase; Ribulose Bisphosphate Carboxylase/Oxygenase (Rubisco) assay kit
Tag
Rubisco
Features & Benefits
Determination of Rubisco activity in plant tissues.
Detailed sample preparation and result calculation methods are provided.
Detailed sample preparation and result calculation methods are provided.
Kit Components
Extraction BufferI
Extraction BufferII
ReagentI
ReagentII
ReagentIII
ReagentIV
Ribulose bisphosphate carboxylase/oxygenase: Rubisco Colorimetric Assay Kit provides a simple method for detecting Rubisco activity in plant tissues. One molecule of ribulose-1,5-diphosphate: RuBP combines with one molecule of CO2 to produce two molecules of 3-phosphoglyceric acid: PGA Glyceraldehyde-3-phosphate can be produced by PGA through the action of additional 3-phosphoglycerate kinase and glyceraldehyde-3-phosphate dehydrogenase, which is accompanied by NADH oxidation to produce NAD+. NADH has a characteristic absorption peak at 340nm, while NAD+ does not. Rubisco activity is calculated by measuring the rate of decrease in light absorption at 340nm.
Extraction BufferII
ReagentI
ReagentII
ReagentIII
ReagentIV
Ribulose bisphosphate carboxylase/oxygenase: Rubisco Colorimetric Assay Kit provides a simple method for detecting Rubisco activity in plant tissues. One molecule of ribulose-1,5-diphosphate: RuBP combines with one molecule of CO2 to produce two molecules of 3-phosphoglyceric acid: PGA Glyceraldehyde-3-phosphate can be produced by PGA through the action of additional 3-phosphoglycerate kinase and glyceraldehyde-3-phosphate dehydrogenase, which is accompanied by NADH oxidation to produce NAD+. NADH has a characteristic absorption peak at 340nm, while NAD+ does not. Rubisco activity is calculated by measuring the rate of decrease in light absorption at 340nm.
Preparation and Storage
Store at -20 degree C and protect from light upon receipt. Kit has a storage time of 6 months form receipt.
Shipping||Gel pack with blue ice.
Shipping||Gel pack with blue ice.
Related Product Information for Ribulose Bisphosphate Carboxylase/Oxygenase (Rubisco) assay kit
Description: Ribulose Bisphosphate Carboxylase/Oxygenase (Rubisco) Colorimetric Assay Kit is designed for detecting Rubisco activity in the sample.
Background: 1,5-ribulose bisphosphate carboxylase/oxygenase (Rubisco, EC 4.1.1.39) is a key enzyme in plant photosynthesis, which not only controls the fixation of CO2, but also controls the distribution of carbon to the Calvin cycle and the photorespiration cycle. Its activity directly affects the photosynthetic rate.
Background: 1,5-ribulose bisphosphate carboxylase/oxygenase (Rubisco, EC 4.1.1.39) is a key enzyme in plant photosynthesis, which not only controls the fixation of CO2, but also controls the distribution of carbon to the Calvin cycle and the photorespiration cycle. Its activity directly affects the photosynthetic rate.
Product Categories/Family for Ribulose Bisphosphate Carboxylase/Oxygenase (Rubisco) assay kit
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Product Notes
The Ribulose Bisphosphate Carboxylase/Oxygenase (Rubisco) (Catalog #AAA9719219) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Ribulose Bisphosphate Carboxylase/Oxygenase (Rubisco), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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