SDS-PAGE
Ribulose-1,5-bisphosphate carboxylase oxygenase Native Protein
Ribulose-1,5-bisphosphate carboxylase oxygenase
Applications
ELISA, Western Blot
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
Ribulose-1; 5-bisphosphate carboxylase oxygenase; RuBisCO; 5-bisphosphate carboxylase oxygenase native protein
Host
Purified from spinach leaf
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Liquid
Applicable Applications for Ribulose-1,5-bisphosphate carboxylase oxygenase native protein
ELISA (EIA), Western Blot (WB)
Peptide
Full length native Ribulose-1,5-bisphosphate carboxylase oxygenase
Storage Buffer
20mMPB pH7.4,1mMDTT,0.1mMEDTA,12.5%glycerol
Preparation and Storage
Aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze / thaw cycles
SDS-PAGE
SDS-PAGE
Related Product Information for Ribulose-1,5-bisphosphate carboxylase oxygenase native protein
Ribulose-1,5-bisphosphate carboxylase oxygenase, most commonly known by the shorter name RuBisCO,is an enzyme involved in the Calvin cycle that catalyzes the first major step of carbon fixation, a process by which the atoms of atmospheric carbon dioxide are made available to organisms in the form of energy-rich molecules such as glucose. RuBisCO catalyzes either the carboxylation or the oxygenation of ribulose-1,5-bisphosphate (also known as RuBP) with carbon dioxide or oxygen. RuBisCO is very important in terms of biological impact because it catalyzes the primary chemical reaction by which inorganic carbon permanently enters the biosphere. Many autotrophic bacteria and archaea fix carbon via the reductive acetyl CoA pathway, the 3-hydroxypropionate cycle or the reverse Krebs cycle, but they make up a relatively minor portion of global net primary production. Phosphoenolpyruvate carboxylase PEPC only temporarily fixes carbon. RuBisCO is also the most abundant protein in leaves, and is considered to be the most abundant protein on Earth.It accounts for 50% of soluble leaf protein in C3 plants (20-30% of total leaf nitrogen) and 30% of soluble leaf protein in C4 plants (5-9% of total leaf nitrogen).Given its important role in the biosphere, there are currently efforts to genetically engineer crop plants so as to contain more efficient RuBisCO.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
ribulose 1,5 bisphosphate carboxylase-oxygenase
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Product Notes
The Ribulose-1,5-bisphosphate carboxylase oxygenase (Catalog #AAA717003) is a Native Protein produced from Purified from spinach leaf and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Ribulose-1,5-bisphosphate carboxylase oxygenase can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Researchers should empirically determine the suitability of the Ribulose-1,5-bisphosphate carboxylase oxygenase for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ribulose-1,5-bisphosphate carboxylase oxygenase, Native Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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