Nitric Oxide Synthase (NOS) Assay Kit | NOS assay kit

Nitric Oxide Synthase (NOS) assay kit (Colormetric method)

Cat. Num. AAA2540419

• Gene Names: NOS3; eNOS; ECNOS
• Synonyms: Nitric Oxide Synthase (NOS); Nitric Oxide Synthase (NOS) assay kit (Colormetric method); NOS assay kit

• Specificity: 100 Assays (Can detect 48 samples without duplication)
• Sequence Length: 118

• Samples: Serum, plasma, tissue homogenate, cells or cell supernatant samples
• Detection Range: 0.2-81.9 U/ml
• Sensitivity: 0.2 U/ml
• Intra-assay Precision: The intra CV is 2.1%.
• Inter-assay Precision: The inter CV is 6%.
• Preparation and Storage: This product has an approximately 2-2.5 month shelf life upon receipt. Please refer to the expiration date on the kit box.

Related Product Information for NOS assay kit

Principle of the Assay: NOS is an enzyme that catalyzes L-arginine and oxygen to produce NO, which can form colored compounds with nucleophilic substances. The activity of NOS can be calculated indirectly by measuring the OD value at 530 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 4262036
• NCBI GeneID: 4846
• Molecular Weight: 67,876 Da
• NCBI Official Full Name: nitric oxide synthase, partial
• NCBI Official Synonym Full Names: nitric oxide synthase 3
• NCBI Official Symbol: NOS3
• NCBI Official Synonym Symbols: eNOS; ECNOS
• NCBI Protein Information: nitric oxide synthase, endothelial
• UniProt Protein Name: Nitric oxide synthase, endothelial
• Protein Family: Nitric oxide synthase
• UniProt Gene Name: NOS3
• UniProt Synonym Gene Names: cNOS; eNOS; NOSIII
• UniProt Entry Name: NOS3_HUMAN

NCBI Description

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016]

Uniprot Description

eNOS: endothelial constitutive nitric oxide synthase. Synthesizes nitric oxide (NO) from arginine and oxygen, which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Induced in humans by caloric restriction. Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN.

Protein type: Amino Acid Metabolism - arginine and proline; EC 1.14.13.39; Oxidoreductase

Chromosomal Location of Human Ortholog: 7q36

Cellular Component: caveola; cytoplasm; cytosol; Golgi membrane; nucleus; plasma membrane

Molecular Function: actin monomer binding; heme binding; nitric-oxide synthase activity; protein binding

Biological Process: arginine catabolic process; blood vessel remodeling; cell redox homeostasis; endothelial cell migration; mitochondrion organization and biogenesis; negative regulation of blood pressure; negative regulation of cell proliferation; negative regulation of muscle hyperplasia; nitric oxide biosynthetic process; nitric oxide mediated signal transduction; positive regulation of angiogenesis; positive regulation of guanylate cyclase activity; positive regulation of vasodilation; regulation of blood vessel size; regulation of nitric-oxide synthase activity; regulation of systemic arterial blood pressure by endothelin; removal of superoxide radicals; smooth muscle hyperplasia

Disease: Alzheimer Disease; Hypertension, Essential; Preeclampsia/eclampsia 1; Stroke, Ischemic