Rabbit PPP2R1B Polyclonal Antibody | anti-PPP2R1B antibody

PPP2R1B Polyclonal Antibody

Cat. Num. AAA3006098

• Gene Names: PPP2R1B; PR65B; PP2A-Abeta
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PPP2R1B; Polyclonal Antibody; PPP2R1B Polyclonal Antibody; Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform; PP2A subunit A isoform PR65-beta; PP2A subunit A isoform R1-beta; PP2A-Abeta; PP2AAbeta; PP2A Abeta; PP2A-APR65beta; PP2A -AR1beta; anti-PPP2R1B antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PPP2R1B polyclonal antibody detects endogenous levels of PPP2R1B protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 556

• Applicable Applications for anti-PPP2R1B antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; ICC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 556-601 of Human PPP2R1B.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of PP2A-Abeta (F590) pAb at 1:1000 dilutionLane1:A2780 whole cell lysate (40ug) Lane2:Beas-2B whole cell lysate (40ug) Lane3:Myla2059 whole cell lysate (40ug) Lane4:3T3-L1 whole cell lysate (40ug))

Related Product Information for anti-PPP2R1B antibody

In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunits have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C. An additional protein phosphatase catalytic subunit, PPX (also known as PP4), is a putative member of a novel PP family. The PP2A family comprises subfamily members PP2Aalpha and PP2Abeta. The PP2A catalytic subunit associates with a variety of regulatory subunits. Regulatory subunits include PP2A-Aalpha and -Abeta, PP2A-Balpha and -Bbeta, PP2A-Calpha and -Cbeta, and PP2A-B56alpha and -B56beta.

NCBI and Uniprot Product Information

• NCBI GI #: 294774574
• NCBI GeneID: 5519
• NCBI Accession #: NP_001171033.1
• NCBI GenBank Nucleotide #: NP_001171033.1
• UniProt Accession #: P30154
• Molecular Weight: ~ 66kDa
• NCBI Official Full Name: serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform isoform d
• NCBI Official Synonym Full Names: protein phosphatase 2 scaffold subunit Abeta
• NCBI Official Symbol: PPP2R1B
• NCBI Official Synonym Symbols: PR65B; PP2A-Abeta
• NCBI Protein Information: serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform
• UniProt Protein Name: Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform
• UniProt Gene Name: PPP2R1B
• UniProt Entry Name: 2AAB_HUMAN

NCBI Description

This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes a beta isoform of the constant regulatory subunit A. Mutations in this gene have been associated with some lung and colon cancers. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]

Uniprot Description

PPP2R1B: The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Belongs to the phosphatase 2A regulatory subunit A family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein phosphatase, regulatory subunit

Chromosomal Location of Human Ortholog: 11q23.2

Cellular Component: lipid raft

Molecular Function: protein binding

Disease: Lung Cancer