Principle of the Assay: MAO can catalysis 4-dimethylambenzylamine to produce p-dimethylaminoben -zaldehyde. p-Dimethylaminobenzaldehyde has a characteristic absorption peak at 355 nm. The activity of MAO can be calculated indirectly by analyzing the production of p-dimethylaminobenzaldehyde.
Background/Introduction: Monoamine oxidase (MAO, EC1.4.3.4) is a "flavin protein" containing alpha-mercaptopropionicacid in the outer membrane of mitochondria o f cells. Its main function in vivo is to catalyze the metabolism of endogenous and exogenous monoamine substances. Under the action of MAO, monoamines are oxidized to produce deamination. MAO is ubiquitous in central nervous system and nerve endings, and it is mainly located on the outer membrane of mitochondria of tissues such as brain, liver and intestinal mucosa.
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Product Notes
The MAO (Catalog #AAA2567677) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Monoamine Oxidase (MAO), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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