MMP-14 assay kit

MMP-14 Inhibitor Screening Kit (Fluorometric)

Cat. Num. AAA846853

• Gene Names: MMP14; MMP-14; MMP-X1; MT-MMP; MT1MMP; MTMMP1; WNCHRS; MT1-MMP; MT-MMP 1
• Synonyms: MMP-14; MMP-14 Inhibitor Screening Kit (Fluorometric); Matrix metalloproteinase-14 Inhibitor Screening Kit; MT1-MMP Inhibitor Screening Kit; MMP-14 assay kit

• Sequence Length: 582

• Application Notes: Screening/studying/characterizing potential MMP-14 inhibitors
• Preparation and Storage: Store at -20 degree C for 12 months.

Typical Standard Curve/Testing Data

Related Product Information for MMP-14 assay kit

Background/Introduction: The Matrix metalloproteinase-14 (MMP-14) (MT1-MMP) belongs to a family of zinc-dependent endopeptidases that are responsible for the degradation of the extracellular matrix (ECM) proteins including collagens, elastins, gelatin, matrix glycoproteins and proteoglycans, during normal development and disease processes. MMPs can be classified into at least four different families of closely related members - collagenases, gelatinases, stromelysins, and membrane-type MMPs (MT-MMPs). MMP-14 belongs to the family of MT-MMPs and is localized on cell surfaces via its transmembrane domain. MMP-14 is expressed in adult lung, placenta, kidney, ovaries, intestine, prostate and spleen along with certain carcinomas. The ability of MMP-14 to degrade type I collagen, and activate pro-MMP-2 and pro-MMP-9 makes it a key enzyme in many physiological and pathological processes such as angiogenesis and tumor invasion. MMP-14 Inhibitor Screening Kit provides a simple, fast and high-throughput adaptable method to screen/study/characterize potential MMP-14 inhibitors. The assay utilizes the ability of MMP-14 to cleave a synthetic MCA-based peptide substrate to release free MCA (7-Methoxycoumarin-4-acetic acid), which can be easily quantified using a fluorometer or fluorescence microplate reader at Ex/Em = 325/420 nm. In the presence of a MMP-14 specific inhibitor, the cleavage of the substrate is reduced/abolished, resulting in decrease or total loss of the MCA fluorescence.

Product Categories/Family for MMP-14 assay kit

MMP

NCBI and Uniprot Product Information

• NCBI GI #: 4826834
• NCBI GeneID: 4323
• NCBI Accession #: NP_004986.1
• NCBI GenBank Nucleotide #: NM_004995.3
• UniProt Accession #: P50281; Q6GSF3; Q92678; A8K5L0
• Molecular Weight: 65,894 Da
• NCBI Official Full Name: matrix metalloproteinase-14 preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 14
• NCBI Official Symbol: MMP14
• NCBI Official Synonym Symbols: MMP-14; MMP-X1; MT-MMP; MT1MMP; MTMMP1; WNCHRS; MT1-MMP; MT-MMP 1
• NCBI Protein Information: matrix metalloproteinase-14
• UniProt Protein Name: Matrix metalloproteinase-14
• UniProt Gene Name: MMP14
• UniProt Synonym Gene Names: MMP-14; MT-MMP 1; MTMMP1; MT1-MMP; MT1MMP

NCBI Description

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion. [provided by RefSeq, Jul 2008]

Uniprot Description

Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Acts as a positive regulator of cell growth and migration via activation of MMP15. Involved in the formation of the fibrovascular tissues in association with pro-MMP2.