GFP Assay Kit
GFP Quantitation Kit
Synonyms
GFP; GFP Quantitation Kit; GFP assay kit
Preparation and Storage
Store all kit components at 4 degree C up to their expiration dates.
Related Product Information for GFP assay kit
Background: Green Fluorescent Protein (GFP) is a spontaneously fluorescent protein originally isolated from the jellyfish Aequorea victoria. Molecular cloning of the GFP gene and its subsequent expression in heterologous systems have established recombinant GFP (rGFP) as a valuable reporter molecule for in vivo visualization of gene expression events in a wide variety of cell types and organisms. Since rGFP requires no additional substrates or cofactors, rGFP fluorescence can be easily detected under fluorescence microscope after expression in either prokaryotic or eukaryotic cells. Most imaging studies of rGFP are qualitative. Quantitative analysis of rGFP level in cells by FACS is time consuming and expensive. GFP Quantitation Kit measures GFP fluorescence in a fluorometer. The quantity of rGFP in sample is determined by comparing its fluorescence reading with that of known recombinant GFP standard curve. The kit has detection sensitivity limit of 100 ng/mL. A proprietary GFP quench solution is also included for determining autofluorescence of cell or tissue sample. Each kit provides sufficient reagents to perform up to 100 assays including standard curve and GFP samples.
Product Categories/Family for GFP assay kit
References
1. Pfeiffer, B. et al. (2012). Using Translational Enhancers to Increase Transgene Expression in Drosophila. PNAS. 109:6626-6631.
2. Wamboldt, Y. et al. (2009). Participation of Leaky Ribosome Scanning in Protein Dual Targeting by Alternative Translation Initiation in Higher Plants. Plant Cell 10.1105/tpc.108.063644.
2. Wamboldt, Y. et al. (2009). Participation of Leaky Ribosome Scanning in Protein Dual Targeting by Alternative Translation Initiation in Higher Plants. Plant Cell 10.1105/tpc.108.063644.
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Product Notes
The GFP (Catalog #AAA168637) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "GFP, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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