Testing Data
CytoSelect Tumor Transendothelial Migration Assay Assay Kit
CytoSelect Tumor Transendothelial Migration Assay
Synonyms
CytoSelect Tumor Transendothelial Migration Assay; CytoSelect Tumor Transendothelial Migration Assay assay kit
Preparation and Storage
CytoTracker Solution and TNFa should be removed from the kit and stored at -20 degree C immediately. Store all other components at 4 degree C.
Testing Data
Testing Data
Testing Data #2
Testing Data #2
Related Product Information for CytoSelect Tumor Transendothelial Migration Assay assay kit
Background: Cancer metastasis comprises several steps. First tumor cells are shed into the blood stream (intravasation), circulating in the blood, and finally transmigrating out of the vessels (extravasation) into a new location in the body. The initial arrest and attachment of tumor cells to vascular endothelium precedes their extravasation from the blood stream and is a crucial step in the tumor metastatic cascade. Tumor cell extravasation is equivalent, in many respects, to the entry of leukocytes into inflammatory tissue. Leukocyte extravasation consists of multiple, consecutive processes including the capture of circulating leukocytes, subsequent leukocyte rolling, arrest, firm adhesion and transmigration. Increasing evidence suggests that tumor cell adhesion to the endothelial lining and transendothelial migration is influenced by endothelial activation or tissue-specific differences in endothelium and depends on the expression of specific cell surface molecules. E-Selectin and Vascular Cell Adhesion Molecule-1 (VCAM-1) appear to play a pivotal role in the tumor-EC interaction. CytoSelect Tumor Transendothelial Migration Assay provides a robust system for the quantitative determination of tumor-endothelium interactions and transmigrations. The kit contains sufficient reagents for the evaluation of 24 assays in a 24-well plate.
Product Categories/Family for CytoSelect Tumor Transendothelial Migration Assay assay kit
References
1. Zen K. and Parkos C. A. (2003) Curr Opin Cell Biol. 15, 557-64.
1. Park, G. B. et al. (2015). Silencing of galectin-3 represses osteosarcoma cell migration and invasion through inhibition of FAK/Src/Lyn activation and beta-catenin expression and increases susceptibility to chemotherapeutic agents. Int J Oncol. 46:185-194.
2. Park, G.B. et al. (2014). The Epstein-Barr virus causes epithelial-mesenchymal transition in human corneal epithelial cells via Syk/Src and Akt/Erk signaling pathways. Invest. Ophthalmol. Vis. Sci. 55:1770-1779.
3. Xu, Z. et al. (2010). Role of pancreatic stellate cells in pancreatic cancer metastasis. Am. J. Pathol., 177:2585-2596.
4. Yang, H. and H.E. Grossniklaus (2010). Consitutive overexpression of pigment epithelium derived factor inhibition of ocular melanoma growth and metastasis. Invest. Ophthalmol. Vis. Sci. 51:28-34.5. Liu, K. et al. (2007). Lentivirus mediated gene transfer of PEDF results in decreased uveal melanoma transendothelial migration. Invest. Opthalmol. Vis. Sci. 48:5244.
1. Park, G. B. et al. (2015). Silencing of galectin-3 represses osteosarcoma cell migration and invasion through inhibition of FAK/Src/Lyn activation and beta-catenin expression and increases susceptibility to chemotherapeutic agents. Int J Oncol. 46:185-194.
2. Park, G.B. et al. (2014). The Epstein-Barr virus causes epithelial-mesenchymal transition in human corneal epithelial cells via Syk/Src and Akt/Erk signaling pathways. Invest. Ophthalmol. Vis. Sci. 55:1770-1779.
3. Xu, Z. et al. (2010). Role of pancreatic stellate cells in pancreatic cancer metastasis. Am. J. Pathol., 177:2585-2596.
4. Yang, H. and H.E. Grossniklaus (2010). Consitutive overexpression of pigment epithelium derived factor inhibition of ocular melanoma growth and metastasis. Invest. Ophthalmol. Vis. Sci. 51:28-34.5. Liu, K. et al. (2007). Lentivirus mediated gene transfer of PEDF results in decreased uveal melanoma transendothelial migration. Invest. Opthalmol. Vis. Sci. 48:5244.
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Product Notes
The CytoSelect Tumor Transendothelial Migration Assay (Catalog #AAA169012) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "CytoSelect Tumor Transendothelial Migration Assay, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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