Typical Standard Curve/Testing Data
Creatinine Assay Kit
Creatinine Assay Kit (urine normalizing assay)
Synonyms
Creatinine; Creatinine Assay Kit (urine normalizing assay); Creatinine Microplate Assay; Creatinine assay kit
Preparation and Storage
1. Store the components of this kit at the temperatures specified on the labels.
2. Unopened reagents are stable until the indicated kit expiration date.
2. Unopened reagents are stable until the indicated kit expiration date.
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data
Related Product Information for Creatinine assay kit
Principle of the Assay: This is a colorimetric assay for the quantitative analysis of creatinine levels in urine. Urinary creatinine reacts with picric acid under alkaline conditions to produce an orange color, which can be quantified by absorption spectroscopy near the 500 nm wavelength. This reaction, known as the Jaffe reaction, also occurs non-specifically with other components in biological fluids. However, the specific color produced with creatine in this reaction is known to degrade rapidly under acidic conditions (Slot et al.). Heinegard and Tiderstrom showed that the difference in color intensity determined before and after the addition of acid is a direct estimate of creatinine concentration in the sample.
Background/Introduction: Creatine (Cr) is produced in the kidney, liver and pancreas, phosphorylated, and transported to the brain and muscle tissue. However, a small proportion of free Cr is converted irreversibly to creatinine (Crn) in the muscular tissue in proportion to the muscle mass. The amount of Crn excreted daily by an individual is relatively constant. Thus, urinary creatinine levels may be used as an index of standardization. 24-h urinary Crn excretion is used to estimate total muscle mass because the rate of non-enzymatic production of Crn from Cr is nearly constant and >90% of the total body Cr is found in muscle tissue. Normal urinary creatinine values for men and women range from 9.7 - 24.7 and 7.9 - 14.2 mmol/24h respectively. Changes in excretion rate may be indicative of impaired renal metabolism.
Background/Introduction: Creatine (Cr) is produced in the kidney, liver and pancreas, phosphorylated, and transported to the brain and muscle tissue. However, a small proportion of free Cr is converted irreversibly to creatinine (Crn) in the muscular tissue in proportion to the muscle mass. The amount of Crn excreted daily by an individual is relatively constant. Thus, urinary creatinine levels may be used as an index of standardization. 24-h urinary Crn excretion is used to estimate total muscle mass because the rate of non-enzymatic production of Crn from Cr is nearly constant and >90% of the total body Cr is found in muscle tissue. Normal urinary creatinine values for men and women range from 9.7 - 24.7 and 7.9 - 14.2 mmol/24h respectively. Changes in excretion rate may be indicative of impaired renal metabolism.
References
1. Slot, C.; (1965) Scand J. Clin. Lab. Invest. 17:381
2. Heinegard, D. and Tiderstrom, G.; (1973) Clin. Chim. Acta 43:305
3. Cook, J.G.H.; (1975) Ann Clin. Biochem. 43:305
4. Young, D.S., Editor; (1990) Effects of drugs on clinical laboratory tests. AACC Press, Washington
5. Wyss, M. and Kaddurah-Daouk, R.; (2000) Phys. Rev. 80:1107
2. Heinegard, D. and Tiderstrom, G.; (1973) Clin. Chim. Acta 43:305
3. Cook, J.G.H.; (1975) Ann Clin. Biochem. 43:305
4. Young, D.S., Editor; (1990) Effects of drugs on clinical laboratory tests. AACC Press, Washington
5. Wyss, M. and Kaddurah-Daouk, R.; (2000) Phys. Rev. 80:1107
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Product Notes
The Creatinine (Catalog #AAA480387) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Creatinine, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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