Typical Testing Data/Standard Curve (for reference only)
(Fig. Typical data of pNA standard curve using Caspase-9 Assay Kit (Colorimetric).)
Caspase-9 Assay Kit | CASP9 assay kit
Caspase-9 Assay Kit (Colorimetric)
Gene Names
CASP9; MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6
Synonyms
Caspase-9; Caspase-9 Assay Kit (Colorimetric); CASP9 assay kit
Sequence Length
266
Application Notes
Caspase 9 Assay Kit (Colorimetric) is based on the hydrolysis of the peptide substrate Ac-LEHD-pNA (acetyl-Leu-Glu-His-Asp p-nitroanilide) by caspase 9, resulting in the release of the p-nitroaniline (pNA), which can be quantified using a spectrophotometer or a microtiter plate reader at at 405 nm.
Preparation and Storage
Stable for at least 12 months at recommended temperature from date of shipment.
Typical Testing Data/Standard Curve (for reference only)
(Fig. Typical data of pNA standard curve using Caspase-9 Assay Kit (Colorimetric).)
Typical Testing Data/Standard Curve (for reference only)
(Fig. Typical data of pNA standard curve using Caspase-9 Assay Kit (Colorimetric).)
Related Product Information for CASP9 assay kit
Description: Caspase-9 Assay Kit (Colorimetric) provides a simple and convenient method for detecting the activity of caspase-9.
Background: Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Mammalian caspases can be subdivided into three functional groups: initiator caspases (Caspase 2, 8, 9 and 10), executioner caspases (Caspase-3, 6 and 7), and inflammatory caspases (Caspase 1, 4, 5, 11 and 12). Caspase-9 (also known as ICE-LAP6 and Mch6) is a member of the caspase-family of cysteine proteases. Caspase-9 is activated by recruitment and dimerization within the Apaf-1 apoptosome. Once recruited, caspase-9 undergoes proteolytic cleavage at Asp315 to yield 35-kDa and 10-kDa fragments. Unlike other caspases, this cleavage event is not required for the catalytic activity of caspase-9. As an initiator caspase, this protease initiates a caspase signaling cascade that results in apoptosis.
Background: Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Mammalian caspases can be subdivided into three functional groups: initiator caspases (Caspase 2, 8, 9 and 10), executioner caspases (Caspase-3, 6 and 7), and inflammatory caspases (Caspase 1, 4, 5, 11 and 12). Caspase-9 (also known as ICE-LAP6 and Mch6) is a member of the caspase-family of cysteine proteases. Caspase-9 is activated by recruitment and dimerization within the Apaf-1 apoptosome. Once recruited, caspase-9 undergoes proteolytic cleavage at Asp315 to yield 35-kDa and 10-kDa fragments. Unlike other caspases, this cleavage event is not required for the catalytic activity of caspase-9. As an initiator caspase, this protease initiates a caspase signaling cascade that results in apoptosis.
Product Categories/Family for CASP9 assay kit
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]
Research Articles on CASP9
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Product Notes
The CASP9 (Catalog #AAA9718959) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Caspase 9 Assay Kit (Colorimetric) is based on the hydrolysis of the peptide substrate Ac-LEHD-pNA (acetyl-Leu-Glu-His-Asp p-nitroanilide) by caspase 9, resulting in the release of the p-nitroaniline (pNA), which can be quantified using a spectrophotometer or a microtiter plate reader at at 405 nm. Researchers should empirically determine the suitability of the CASP9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Caspase-9, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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