Typical Testing Data/Standard Curve (for reference only)
(Fig. Typical data of pNA standard curve using Caspase-2 Assay Kit (Colorimetric).)
Caspase-2 Assay Kit | Casp2 assay kit
Caspase-2 Assay Kit (Colorimetric)
Synonyms
Caspase-2; Caspase-2 Assay Kit (Colorimetric); Casp2 assay kit
Sequence Length
452
Application Notes
Caspase 2 Assay Kit (Colorimetric) is based on the hydrolysis of the peptide substrate Ac-VDQQD-pNA (acetyl-Val-Asp-Gln-Gln-Asp p-nitroanilide) by caspase 2, resulting in the release of the p-nitroaniline (pNA), which can be quantified using a spectrophotometer or a microtiter plate reader at at 405 nm.
Preparation and Storage
Stable for at least 12 months at recommended temperature from date of shipment.
Typical Testing Data/Standard Curve (for reference only)
(Fig. Typical data of pNA standard curve using Caspase-2 Assay Kit (Colorimetric).)
Typical Testing Data/Standard Curve (for reference only)
(Fig. Typical data of pNA standard curve using Caspase-2 Assay Kit (Colorimetric).)
Related Product Information for Casp2 assay kit
Description: Caspase-2 Assay Kit (Colorimetric) provides a simple and convenient method for detecting the activity of caspase-2.
Background: Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Mammalian caspases can be subdivided into three functional groups: initiator caspases (Caspase 2, 8, 9 and 10), executioner caspases (Caspase-3, 6 and 7), and inflammatory caspases (Caspase 1, 4, 5, 11 and 12). Caspase 2 belongs to a family of cysteine proteases that cleave proteins only at an amino acid following an aspartic acid residue. Within this family, caspase 2 is part of the Ich-1 subfamily, one of the most conserved caspases in different species of animal. It has a similar amino acid sequence to initiator caspases, including caspase 1, caspase 4, caspase 5, and caspase 9. It is produced as a zymogen, which contains a long pro-domain that is similar to that of caspase 9 and contains a protein interaction domain known as a CARD domain. Pro-caspase-2 contains two subunits, p19 and p12.
Background: Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Mammalian caspases can be subdivided into three functional groups: initiator caspases (Caspase 2, 8, 9 and 10), executioner caspases (Caspase-3, 6 and 7), and inflammatory caspases (Caspase 1, 4, 5, 11 and 12). Caspase 2 belongs to a family of cysteine proteases that cleave proteins only at an amino acid following an aspartic acid residue. Within this family, caspase 2 is part of the Ich-1 subfamily, one of the most conserved caspases in different species of animal. It has a similar amino acid sequence to initiator caspases, including caspase 1, caspase 4, caspase 5, and caspase 9. It is produced as a zymogen, which contains a long pro-domain that is similar to that of caspase 9 and contains a protein interaction domain known as a CARD domain. Pro-caspase-2 contains two subunits, p19 and p12.
Product Categories/Family for Casp2 assay kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
caspase-2
NCBI Official Synonym Full Names
caspase 2
NCBI Official Symbol
Casp2
NCBI Protein Information
caspase-2
UniProt Protein Name
Caspase-2
Protein Family
UniProt Gene Name
Casp2
UniProt Synonym Gene Names
Ich1; CASP-2
UniProt Entry Name
CASP2_RAT
NCBI Description
may play a role in proteolysis and programmed cell death [RGD, Feb 2006]
Research Articles on Casp2
Similar Products
Product Notes
The Casp2 casp2 (Catalog #AAA9718954) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Caspase 2 Assay Kit (Colorimetric) is based on the hydrolysis of the peptide substrate Ac-VDQQD-pNA (acetyl-Val-Asp-Gln-Gln-Asp p-nitroanilide) by caspase 2, resulting in the release of the p-nitroaniline (pNA), which can be quantified using a spectrophotometer or a microtiter plate reader at at 405 nm. Researchers should empirically determine the suitability of the Casp2 casp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Caspase-2, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
