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Typical Testing Data/Standard Curve (for reference only)

General Alpha-Amylase Assay Kit | AMY2A assay kit

Alpha-Amylase Microplate Assay Kit

Gene Names
AMY2A; PA; AMY2
Reactivity
General
Applications
Functional Assay
Synonyms
Alpha-Amylase; Alpha-Amylase Microplate Assay Kit; EC 3.2.1.1; Alpha-amylase; Glycogenase; A-Amylase; AMY2A assay kit
Ordering
For Research Use Only!
Reactivity
General
Sequence Length
511
Applicable Applications for AMY2A assay kit
Functional Assay
Samples
Serum, Plasma, Urine, Saliva, Cell Culture, Tissue Extracts, Cell Lysate, Other Biological Fluids Samples
Detection Range
1 umol/L-1000 umol/L
Preparation and Storage
Shipped and store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for AMY2A assay kit
Background/Introduction: Ammonia (NH3) or its ion form ammonium (NH4+) is an important source of nitrogen for living systems. It is synthesized through amino acid metabolism and is toxic when present at high concentrations. In the liver, ammonia is converted to urea through the urea cycle. Elevated levels of ammonia in the blood (hyperammonemia) have been found in liver dysfunction (cirrhosis), while hypoammonemia has been associated with defects in the urea cycle enzymes (e.g. ornithine transcarbamylase). Ammonia/Ammonium Microplate Assay Kit is designed to directly measure NH3 and NH4+ in a variety of samples. In the assay, ammonia reacts with hypochlorous acid, which is determined at 620nm, is directly proportional to the Ammonia/Ammonium concentration in the sample.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
279
Molecular Weight
30,302 Da
NCBI Official Full Name
alpha-amylase
NCBI Official Synonym Full Names
amylase, alpha 2A (pancreatic)
NCBI Official Symbol
AMY2A
NCBI Official Synonym Symbols
PA; AMY2
NCBI Protein Information
pancreatic alpha-amylase
UniProt Protein Name
Pancreatic alpha-amylase
Protein Family
UniProt Gene Name
AMY2A
UniProt Synonym Gene Names
PA
UniProt Entry Name
AMYP_HUMAN

NCBI Description

This gene encodes a member of the alpha-amylase family of proteins. Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, catalyzing the first step in digestion of dietary starch and glycogen. This gene and several family members are present in a gene cluster on chromosome 1. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jan 2015]

Uniprot Description

AMY2A: Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jul 2008]

Protein type: EC 3.2.1.1; Hydrolase; Carbohydrate Metabolism - starch and sucrose; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1p21

Cellular Component: extracellular region; extracellular space

Molecular Function: alpha-amylase activity; calcium ion binding; chloride ion binding

Biological Process: carbohydrate catabolic process; carbohydrate metabolic process; polysaccharide digestion

Research Articles on AMY2A

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Product Notes

The General AMY2A amy2a (Catalog #AAA8243219) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA8243219 Assay Kit recognizes General AMY2A. AAA Biotech's Alpha-Amylase can be used in a range of immunoassay formats including, but not limited to, Functional Assay. Researchers should empirically determine the suitability of the AMY2A amy2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alpha-Amylase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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