Testing Data
HIF1B recombinant protein
HIF1B Protein
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Testing Data
Testing Data
Scientific Background: The transcription factor Aryl hydrocarbon receptor nuclear translocator (ARNT), alternatively named Hypoxia-inducible factor (HIF)-1beta, is part of the Hypoxia-inducible factor (HIF) pathway which mediates cellular adaptations to hypoxia and promotes tumour progression. Unlike HIF-1alpha that is regulated in an oxygen-dependent manner, HIF-1beta is constitutively expressed. It has been shown that some tumour cells are capable to elevate HIF-1beta in response to hypoxia to for a clonal advantage. HIF-1beta binds HIF-1alpha, and forms the transcriptional active HIF-1 complex in the nucleus.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. Chromosomal translocation of this locus with the ETV6 (ets variant 6) gene on chromosome 12 have been described in leukemias. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2013]
Uniprot Description
ARNT: a bHLH transcription factor that requires dimerization with another bHLH protein for efficient DNA binding. A binding partner for the Ah (dioxin) nuclear receptor. Required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. A binding partner for HIF1A or HIF2A, transcription factors that regulates transcription from RNA polymerase II promoter in the adaptive response to hypoxia and oxidative stress. This complex activates target genes that limit oxygen consumption. HIF-1alpha may have nontranscriptional activities that inhibit DNA replication and cell proliferation when oxygen becomes scarce. Forms heterodimers with other bHLH proteins. Interacts with TACC3. Three isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Motility/polarity/chemotaxis; Transcription factor
Chromosomal Location of Human Ortholog: 1q21.3
Cellular Component: nuclear body; nucleoplasm; nucleus
Molecular Function: aryl hydrocarbon receptor binding; protein binding; protein heterodimerization activity; RNA polymerase II transcription factor activity, enhancer binding; sequence-specific DNA binding; transcription factor activity; transcription factor binding
Biological Process: mRNA transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; positive regulation of erythrocyte differentiation; positive regulation of glycolysis; positive regulation of hormone biosynthetic process; positive regulation of transcription, DNA-dependent; positive regulation of vascular endothelial growth factor receptor signaling pathway; regulation of transcription from RNA polymerase II promoter in response to oxidative stress; response to hypoxia; transcription, DNA-dependent; xenobiotic metabolic process
Research Articles on HIF1B
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Product Notes
The HIF1B arnt (Catalog #AAA515507) is a Recombinant Protein produced from Sf9 insect cells using baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Full length. AAA Biotech's HIF1B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the HIF1B arnt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HIF1B, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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