Rabbit ERK1 Polyclonal Antibody | anti-ERK1 antibody

Phospho-ERK1 (Thr202/Tyr204)/ERK2 (Thr185/Tyr187) Rabbit pAb

Cat. Num. AAA4757701

• Gene Names: MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: Affinity purified
• Synonyms: ERK1; Polyclonal Antibody; Phospho-ERK1 (Thr202/Tyr204)/ERK2 (Thr185/Tyr187) Rabbit pAb; anti-ERK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Purity/Purification: Affinity purified
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Applicable Applications for anti-ERK1 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IHC1: 1:50-1:100; IP: 1:50-1:100
• Immunogen: A synthetic phosphorylated peptide around T185/Y187 of human ERK2 (NP_002736.3).
• Conjugation: Unconjugated
• Modification: Phosphoralated
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb Western blot analysis of extracts of various cell lines, using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 antibody at 1:5000 dilution or ERK1/2 antibody .293T cells and C6 cells were treated by ATP(5 mM) at 30 degree C for 1 hour. NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37 degree C for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Exposure time: 10s.)

Immunohistochemistry (IHC)

(Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb Immunohistochemistry of paraffin-embedded rat lung using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb Immunohistochemistry of paraffin-embedded rat testis using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb Immunohistochemistry of paraffin-embedded rat brain using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb Immunohistochemistry of paraffin-embedded rat spleen using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb Immunohistochemistry of paraffin-embedded rat heart using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb Immunohistochemistry of paraffin-embedded human tonsil using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb Immunohistochemistry of paraffin-embedded human breast cancer using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb Immunohistochemistry of paraffin-embedded human gastric cancer using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb Immunohistochemistry of paraffin-embedded human uterine cancer using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb Immunohistochemistry of paraffin-embedded mouse liver using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb Immunohistochemistry of paraffin-embedded mouse testis using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb Immunohistochemistry of paraffin-embedded mouse brain using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb Immunohistochemistry of paraffin-embedded mouse spleen using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody at dilution of 1:100 (40x lens).)

Product Categories/Family for anti-ERK1 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 91718897
• NCBI GeneID: 5595
• NCBI Accession #: NP_001035145.1
• NCBI GenBank Nucleotide #: NM_001040056.2
• UniProt Accession #: P27361; P28482
• Molecular Weight: 43,136 Da
• NCBI Official Full Name: mitogen-activated protein kinase 3 isoform 2
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 3
• NCBI Official Symbol: MAPK3
• NCBI Official Synonym Symbols: ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
• NCBI Protein Information: mitogen-activated protein kinase 3; MAPK 1; MAP kinase isoform p44; insulin-stimulated MAP2 kinase; extracellular signal-related kinase 1; extracellular signal-regulated kinase 1; microtubule-associated protein 2 kinase
• UniProt Protein Name: Mitogen-activated protein kinase 3
• UniProt Gene Name: MAPK3
• UniProt Synonym Gene Names: ERK1; PRKM3; MAP kinase 3; MAPK 3; ERK-1; p44-MAPK
• UniProt Entry Name: MK03_HUMAN

NCBI Description

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

ERK1: a serine/threonine kinase of the GMGC group that plays a critical role in the regulation of cell growth and differentiation. ERK1 (MAPK3) and ERK2 (MAPK1) play central roles in MAPK cascades and are activated by a wide variety of extracellular signals including growth and neurotrophic factors, cytokines, hormones and neurotransmitters. Depending on the cellular context, MAPK cascades mediate diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. MAPK cascades also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine residues at the sequence T*EY* by upstream MAP kinase kinases, MEK1 and -2. Phosphorylation of both the threonine and tyrosine are required for activity. This phosphorylation causes dramatic conformational changes, which enable full activation and interaction of MAPK1/ERK2 with its substrates.

Protein type: Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.24; Kinase, protein; CMGC group; MAPK family; ERK subfamily; MAPK/ERK subfamily

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: Golgi apparatus; focal adhesion; mitochondrion; early endosome; nuclear envelope; pseudopodium; caveola; cytosol; nucleoplasm; microtubule cytoskeleton; cytoskeleton; late endosome; nucleus

Molecular Function: MAP kinase activity; protein binding; phosphotyrosine binding; ATP binding; phosphatase binding

Biological Process: axon guidance; viral reproduction; nerve growth factor receptor signaling pathway; positive regulation of histone phosphorylation; DNA damage induced protein phosphorylation; activation of MAPKK activity; apoptosis; activation of MAPK activity; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; sensory perception of pain; protein amino acid phosphorylation; toll-like receptor 10 signaling pathway; BMP signaling pathway; toll-like receptor 5 signaling pathway; response to exogenous dsRNA; regulation of transcription factor activity; small GTPase mediated signal transduction; lipopolysaccharide-mediated signaling pathway; toll-like receptor 4 signaling pathway; epidermal growth factor receptor signaling pathway; platelet activation; fibroblast growth factor receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; cytokine and chemokine mediated signaling pathway; MAPKKK cascade; transcription from RNA polymerase I promoter; cell cycle; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; regulation of stress-activated MAPK cascade; organ morphogenesis; cartilage development; Ras protein signal transduction; toll-like receptor signaling pathway; insulin receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of histone acetylation; gene expression; positive regulation of protein amino acid phosphorylation; toll-like receptor 9 signaling pathway; vascular endothelial growth factor receptor signaling pathway; blood coagulation; transcription initiation from RNA polymerase I promoter; phosphorylation; regulation of cytoskeleton organization and biogenesis