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SDS-Page

ARL2 recombinant protein

ARL2, 1-184aa, Human, His tag, E Coli

Gene Names
ARL2; ARFL2
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
ARL2; 1-184aa; Human; His tag; E Coli; ADP-ribosylation factor-like protein 2; ARFL2; ARL2 recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 0.1 mM PMSF, 1mM DTT, 40% glycerol
Concentration
0.25 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGLLTILKKM KQKERELRLL MLGLDNAGKT TILKKFNGED IDTISPTLGF NIKTLEHRGF KLNIWDVGGQ KSLRSYWRNY FESTDGLIWV VDSADRQRMQ DCQRELQSLL VEERLAGATL LIFANKQDLP GALSSNAIRE ALELDSIRSH HWCIQGCSAV TGENLLPGID WLLDDISSRI FTAD
Sequence Length
184
Applicable Applications for ARL2 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

SDS-Page

SDS-Page
Related Product Information for ARL2 recombinant protein
ARL2 belongs to the ADP-ribosylation factor (ARF) family. This protein functions as a component of a secretory pathway that is involved in the Ca2-dependent release of acetylcholine. It plays a role in the folding of tubule proteins, thereby playing an important role in microtubule dynamics and cell cycle progression. unlike other members of the ARF family, ARL2 does not act as an activator of the catalytic subunit of cholera toxin. Recombinant human ARL2 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.
Product Categories/Family for ARL2 recombinant protein
References
Smith DF., et al. (2010) Mol Biochem Parasitol. 173 (2):123-31.; Dumontet C., et al (2009) PLoS One. 4 (10):e7478.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
402
Molecular Weight
23.0 kDa (204aa), confirmed by MALDI-TOF
NCBI Official Full Name
ADP-ribosylation factor-like protein 2
NCBI Official Synonym Full Names
ADP ribosylation factor like GTPase 2
NCBI Official Symbol
ARL2
NCBI Official Synonym Symbols
ARFL2
NCBI Protein Information
ADP-ribosylation factor-like protein 2
UniProt Protein Name
ADP-ribosylation factor-like protein 2
UniProt Gene Name
ARL2

NCBI Description

This gene encodes a small GTP-binding protein of the RAS superfamily which functions as an ADP-ribosylation factor (ARF). The encoded protein is one of a functionally distinct group of ARF-like genes. [provided by RefSeq, Jul 2008]

Uniprot Description

Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. Regulates formation of new microtubules and centrosome integrity. Prevents the TBCD-induced microtubule destruction. Participates in association with TBCD, in the disassembly of the apical junction complexes. Antagonizes the effect of TBCD on epithelial cell detachment and tight and adherens junctions disassembly. Together with ARL2, plays a role in the nuclear translocation, retention and transcriptional activity of STAT3. Component of a regulated secretory pathway involved in Ca2+-dependent release of acetylcholine. Required for normal progress through the cell cycle.

Research Articles on ARL2

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Product Notes

The ARL2 arl2 (Catalog #AAA203986) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's ARL2 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the ARL2 arl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGLLTILKKM KQKERELRLL MLGLDNAGKT TILKKFNGED IDTISPTLGF NIKTLEHRGF KLNIWDVGGQ KSLRSYWRNY FESTDGLIWV VDSADRQRMQ DCQRELQSLL VEERLAGATL LIFANKQDLP GALSSNAIRE ALELDSIRSH HWCIQGCSAV TGENLLPGID WLLDDISSRI FTAD. It is sometimes possible for the material contained within the vial of "ARL2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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