ARF4 recombinant protein

ARF4, 1-180aa, Human, His tag, E Coli

Cat. Num. AAA203759

• Gene Names: ARF4; ARF2
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: ARF4; 1-180aa; Human; His tag; E Coli; ADP-ribosylation factor 4; ARF2; ARF4 recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 0.2M NaCl, 5mM DTT, 20% glycerol
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMGLTIS SLFSRLFGKK QMRILMVGLD AAGKTTILYK LKLGEIVTTI PTIGFNVETV EYKNICFTVW DVGGQDRIRP LWKHYFQNTQ GLIFVVDSND RERIQEVADE LQKMLLVDEL RDAVLLLFAN KQDLPNAMAI SEMTDKLGLQ SLRNRTWYVQ ATCATQGTGL YEGLDWLSNE LSKR
• Sequence Length: 180

• Applicable Applications for ARF4 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for ARF4 recombinant protein

ARF4 is a member of the ARF gene family whose members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The ARF proteins include 5 ARF proteins and 11 ARF-like proteins and constitute one family of the RAS superfamily. They are categorized as class I, class II and class III; this gene is a class II member. Recombinant human ARF4 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for ARF4 recombinant protein

Signal Transduction

References

Mazelova J., et al. (2009) EMBO J. 28 (3):183-92.; Woo IS., et al. (2009) Cancer Lett. 276 (1):53-60.;

NCBI and Uniprot Product Information

• NCBI GI #: 4502205
• NCBI GeneID: 378
• NCBI Accession #: NP_001651
• NCBI GenBank Nucleotide #: NM_001660.3
• UniProt Accession #: P18085; P21371; B2R7J7
• Molecular Weight: 23.0 kDa (204aa), confirmed by MALDI-TOF
• NCBI Official Full Name: ADP-ribosylation factor 4
• NCBI Official Synonym Full Names: ADP ribosylation factor 4
• NCBI Official Symbol: ARF4
• NCBI Official Synonym Symbols: ARF2
• NCBI Protein Information: ADP-ribosylation factor 4
• UniProt Protein Name: ADP-ribosylation factor 4
• Protein Family: Auxin response factor
• UniProt Gene Name: ARF4
• UniProt Synonym Gene Names: ARF2

NCBI Description

This gene is a member of the human ARF gene family whose members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 5 ARF proteins and 11 ARF-like proteins and constitute one family of the RAS superfamily. The ARF proteins are categorized as class I, class II and class III; this gene is a class II member. The members of each class share a common gene organization. The ARF4 gene spans approximately 12kb and contains six exons and five introns. This gene is the most divergent member of the human ARFs. Conflicting map positions at 3p14 or 3p21 have been reported for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.