E3 ubiquitin-protein ligase ARIH1 (ARIH1) Recombinant Protein | ARIH1 recombinant protein

Recombinant Human E3 ubiquitin-protein ligase ARIH1 (ARIH1)

Cat. Num. AAA1439772

• Gene Names: ARIH1; ARI; HARI; HHARI; UBCH7BP
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: E3 ubiquitin-protein ligase ARIH1 (ARIH1); Recombinant Human E3 ubiquitin-protein ligase ARIH1 (ARIH1); ARIH1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-557, Full length protein
• Sequence: MDSDEGYNYEFDEDEECSEEDSGAEEEEDEDDDEPDDDTLDLGEVELVEPGLGVGGERDGLLCGETGGGGGSALGPGGGGGGGGGGGGGGPGHEQEEDYRYEVLTAEQILQHMVECIREVNEVIQNPATITRILLSHFNWDKEKLMERYFDGNLEKLFAECHVINPSKKSRTRQMNTRSSAQDMPCQICYLNYPNSYFTGLECGHKFCMQCWSEYLTTKIMEEGMGQTISCPAHGCDILVDDNTVMRLITDSKVKLKYQHLITNSFVECNRLLKWCPAPDCHHVVKVQYPDAKPVRCKCGRQFCFNCGENWHDPVKCKWLKKWIKKCDDDSETSNWIAANTKECPKCHVTIEKDGGCNHMVCRNQNCKAEFCWVCLGPWEPHGSAWYNCNRYNEDDAKAARDAQERSRAALQRYLFYCNRYMNHMQSLRFEHKLYAQVKQKMEEMQQHNMSWIEVQFLKKAVDVLCQCRATLMYTYVFAFYLKKNNQSIIFENNQADLENATEVLSGYLERDISQDSLQDIKQKVQDKYRYCESRRRVLLQHVHEGYEKDLWEYIED
• Sequence Length: 557

• Species: Human
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 187761373
• NCBI GeneID: 25820
• NCBI Accession #: NP_005735.2
• NCBI GenBank Nucleotide #: NM_005744.3
• UniProt Accession #: Q9Y4X5; O76026; Q9H3T6; Q9UEN0; Q9UP39; B2R6U3
• Molecular Weight: 64,118 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase ARIH1
• NCBI Official Synonym Full Names: ariadne RBR E3 ubiquitin protein ligase 1
• NCBI Official Symbol: ARIH1
• NCBI Official Synonym Symbols: ARI; HARI; HHARI; UBCH7BP
• NCBI Protein Information: E3 ubiquitin-protein ligase ARIH1
• UniProt Protein Name: E3 ubiquitin-protein ligase ARIH1
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: ARIH1
• UniProt Synonym Gene Names: ARI-1

Uniprot Description

E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3 (PubMed:15236971, PubMed:21532592, PubMed:24076655, PubMed:27565346, PubMed:23707686). Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-RING ubiquitin ligase (CRL) complexes and initiating ubiquitination of CRL substrates: associates with CRL complexes and specifically mediates addition of the first ubiquitin on CRLs targets (PubMed:27565346). The initial ubiquitin is then elongated by CDC34/UBE2R1 and UBE2R2 (PubMed:27565346). E3 ubiquitin-protein ligase activity is activated upon binding to neddylated cullin-RING ubiquitin ligase complexes (PubMed:24076655, PubMed:27565346). Plays a role in protein translation in response to DNA damage by mediating ubiquitination of EIF4E2, the consequences of EIF4E2 ubiquitination are however unclear (PubMed:25624349). According to a report, EIF4E2 ubiquitination leads to promote EIF4E2 cap-binding and protein translation arrest (PubMed:25624349). According to another report EIF4E2 ubiquitination leads to its subsequent degradation (PubMed:14623119). Acts as the ligase involved in ISGylation of EIF4E2 (PubMed:17289916).