Ba(E Coli-k12) Ornithine carbamoyltransferase chain F Recombinant Protein | argF recombinant protein

Recombinant Ba(E Coli-k12) Ornithine carbamoyltransferase chain F

Cat. Num. AAA953794

• Gene Names: argF; Arg5; argD; ECK0274; JW0266
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Ba(E Coli-k12) Ornithine carbamoyltransferase chain F; Recombinant Ba(E Coli-k12) Ornithine carbamoyltransferase chain F; OTCase-2; argF recombinant protein

• Host: E Coli
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Liquid containing glycerol
• Sequence Positions: 2-334aa; Full Length
• Sequence: SDLYKKHFLKLLDFTPAQFTSLLTLAAQLKADKKNGKEVQKLTGKNIALIFEKDSTRTRCSFEVAAFDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYDGIQYRGHGQEVVETLAQYAGVPVWNGLTNEFHPTQLLADLMTMQEHLPGKAFNEMTLVYAGDARNNMGNSMLEAAALTGLDLRLLAPKACWPEESLVAECSALAEKHGGKITLTEDVAAGVKGADFIYTDVWVSMGEAKEKWAERIALLRGYQVNAQMMALTDNPNVKFLHCLPAFHDDQTTLGKQMAKEFDLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMMATLGE
• Sequence Length: 334

• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for argF recombinant protein

Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline, which is a substrate for argininosuccinate synthetase, the enzyme involved in the final step in arginine biosynthesis.

References

Evolutionary divergence of genes for ornithine and aspartate carbamoyl-transferases -- complete sequence and mode of regulation of the Escherichia coli argF gene; comparison of argF with argI and pyrB.van Vliet F., Cunin R., Jacobs A., Piette J., Gigot D., Lauwereys M., Pierard A., Glansdorff N.Nucleic Acids Res. 12:6277-6289(1984) Sequence of minutes 4-25 of Escherichia coli.Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997) Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006) Immunological and structural relatedness of catabolic ornithine carbamoyltransferases and the anabolic enzymes of enterobacteria.Falmagne P., Portetelle D., Stalon V.J. Bacteriol. 161:714-719(1985) The dual genetic control of ornithine carbamolytransferase in Escherichia coli. A case of bacterial hybrid enzymes.Legrain C., Halleux P., Stalon V., Glansdorff N.Eur. J. Biochem. 27:93-102(1972) Escherichia coli ornithine carbamolytransferase isoenzymes evolutionary significance and the isolation of lambdaargF and lambdaargI transducing bacteriophages.Legrain C., Stalon V., Glansdorff N.J. Bacteriol. 128:35-38(1976)

NCBI and Uniprot Product Information

• NCBI GI #: 16128258
• NCBI GeneID: 944844
• NCBI Accession #: NP_414807.1
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P06960; P78308; Q2MCE9
• Molecular Weight: 40.7 kDa
• NCBI Official Full Name: ornithine carbamoyltransferase 2, chain F; CP4-6 prophage
• NCBI Official Symbol: argF
• NCBI Official Synonym Symbols: Arg5; argD; ECK0274; JW0266
• NCBI Protein Information: ornithine carbamoyltransferase 2, chain F; CP4-6 prophage
• UniProt Protein Name: Ornithine carbamoyltransferase chain F
• Protein Family: Ornithine carbamoyltransferase
• UniProt Gene Name: argF
• UniProt Entry Name: OTC2_ECOLI

NCBI Description

argI and argF have same enzyme activity. [More information is available at EcoGene: EG10067]. In the test tube, not in the cell, a family of four isoenzymes forms by assortment of two closely similar polypeptides, the chain F-monomer and chain I-monomer, to give active trimers FFF, FFI, FII, and III. [More information is available at EcoCyc: EG10067].

Uniprot Description

Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline, which is a substrate for argininosuccinate synthetase, the enzyme involved in the final step in arginine biosynthesis.