Ba(E Coli-k12) KHG/KDPG aldolase Recombinant Protein | eda recombinant protein

Recombinant Ba(E Coli-k12) KHG/KDPG aldolase

• Gene Names: eda; ECK1851; hga; JW1839; kdgA; kga
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Ba(E Coli-k12) KHG/KDPG aldolase; Recombinant Ba(E Coli-k12) KHG/KDPG aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase (EC:4.1.2.14); 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase; eda recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-213
• Sequence: MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVLEVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLKAATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCPTGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL
• Sequence Length: 213

• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-Page

Related Product Information for eda recombinant protein

Involved in the degradation of glucose via the Entner-Doudoroff pathway. Catalyzes the reversible, stereospecific retro-aldol cleavage of 2-Keto-3-deoxy-6-phosphogluconate (KDPG) to pyruvate and D-glyceraldehyde-3-phosphate. In the synthetic direction, it catalyzes the addition of pyruvate to electrophilic aldehydes with si-facial selectivity. It accepts some nucleophiles other than pyruvate, including 2-oxobutanoate, phenylpyruvate, and fluorobutanoate. It has a preference for the S-configuration at C2 of the electrophile.

References

The complete amino acid sequence and identification of the active-site arginine peptide of Escherichia coli 2-keto-4-hydroxyglutarate aldolase.Vlahos C.J., Dekker E.E.J. Biol. Chem. 263:11683-11691(1988) Cloning, nucleotide sequence, overexpression, and inactivation of the Escherichia coli 2-keto-4-hydroxyglutarate aldolase gene.Patil R.V., Dekker E.E.J. Bacteriol. 174:102-107(1992) Molecular characterization of the Entner-Doudoroff pathway in Escherichia coli sequence analysis and localization of promoters for the edd-eda operon.Egan S.E., Fliege R., Tong S., Shibata A., Wolf R.E. Jr., Conway T.J. Bacteriol. 174:4638-4646(1992) Purine and one-carbon metabolism in Escherichia coli K12 DNA sequence of a second GAR transformylase.Smith J.M., Nygaard P. Sequence of the Escherichia coli K-12 edd and eda genes of the Entner-Doudoroff pathway.Carter A.T., Pearson B.M., Dickinson J.R., Lancashire W.E.Gene 130:155-156(1993) A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map.Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T.DNA Res. 3:379-392(1996) The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997) Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006) Active-site residues of 2-keto-4-hydroxyglutarate aldolase from Escherichia coli. Bromopyruvate inactivation and labeling of glutamate 45.Vlahos C.J., Dekker E.E.J. Biol. Chem. 265:20384-20389(1990) Escherichia coli proteome analysis using the gene-protein database.VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.Electrophoresis 18:1243-1251(1997) Characterization and crystal structure of Escherichia coli KDPGal aldolase.Walters M.J., Srikannathasan V., McEwan A.R., Naismith J.H., Fierke C.A., Toone E.J.Bioorg. Med. Chem. 16:710-720(2008) Covalent intermediate trapped in 2-keto-3-deoxy-6-phosphogluconate (KDPG) aldolase structure at 1.95-A resolution.Allard J., Grochulski P., Sygusch J.Proc. Natl. Acad. Sci. U.S.A. 98:3679-3684(2001) Directed evolution of a new catalytic site in 2-keto-3-deoxy-6-phosphogluconate aldolase from Escherichia coli.Wymer N., Buchanan L.V., Henderson D., Mehta N., Botting C.H., Pocivavsek L., Fierke C.A., Toone E.J., Naismith J.H.Structure 9:1-9(2001) Mechanism of the Class I KDPG aldolase.Fullerton S.W., Griffiths J.S., Merkel A.B., Cheriyan M., Wymer N.J., Hutchins M.J., Fierke C.A., Toone E.J., Naismith J.H.Bioorg. Med. Chem. 14:3002-3010(2006)

NCBI and Uniprot Product Information

• NCBI GI #: 16129803
• NCBI GeneID: 946367
• NCBI Accession #: NP_416364.1
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P0A955; P10177
• Molecular Weight: 26.4kD
• NCBI Official Full Name: KHG/KDPG aldolase; 2-dehydro-3-deoxy-phosphogluconate/4-hydroxy-2-oxoglutarate aldolase
• NCBI Official Symbol: eda
• NCBI Official Synonym Symbols: ECK1851; hga; JW1839; kdgA; kga
• UniProt Protein Name: KHG/KDPG aldolase
• Protein Family: Ectodysplasin
• UniProt Gene Name: eda
• UniProt Synonym Gene Names: hga; kdgA; KHG-aldolase; KDPG-aldolase
• UniProt Entry Name: ALKH_ECOLI

NCBI Description

Eda: [More information is available at EcoCyc: EG10256].

Uniprot Description

Involved in the degradation of glucose via the Entner-Doudoroff pathway. Catalyzes the reversible, stereospecific retro-aldol cleavage of 2-Keto-3-deoxy-6-phosphogluconate (KDPG) to pyruvate and D-glyceraldehyde-3-phosphate. In the synthetic direction, it catalyzes the addition of pyruvate to electrophilic aldehydes with si-facial selectivity. It accepts some nucleophiles other than pyruvate, including 2-oxobutanoate, phenylpyruvate, and fluorobutanoate. It has a preference for the S-configuration at C2 of the electrophile.