Ba(E Coli-k12) Glutamate decarboxylase alpha Recombinant Protein | gadA recombinant protein

Recombinant Ba(E Coli-k12) Glutamate decarboxylase alpha

Cat. Num. AAA1235820

• Gene Names: gadA; ECK3502; gadS; JW3485
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Ba(E Coli-k12) Glutamate decarboxylase alpha; Recombinant Ba(E Coli-k12) Glutamate decarboxylase alpha; gadA recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-466aa; Full Length
• Sequence: MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQIINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT
• Sequence Length: 466

• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for gadA recombinant protein

Converts glutamate to gamma-aminobutyrate (GABA), consuming one intracellular proton in the reaction. The gad system helps to maintain a near-neutral intracellular pH when cells are exposed to extrely acidic conditions. The ability to survive transit through the acidic conditions of the stomach is essential for successful colonization of the mammalian host by commensal and pathogenic bacteria.

References

Escherichia coli has two homologous glutamate decarboxylase genes that map to distinct loci.Smith D.K., Kassam T., Singh B., Elliott J.F.J. Bacteriol. 174:5820-5826(1992) The amino acid sequence of glutamate decarboxylase from Escherichia coli. Evolutionary relationship between mammalian and bacterial enzymes.Maras B., Sweeney G., Barra D., Bossa F., John R.A.Eur. J. Biochem. 204:93-98(1992) Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes.Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.Nucleic Acids Res. 22:2576-2586(1994) The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997) Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006) Expression of the Escherichia coli dimorphic glutamic acid decarboxylases is regulated by the nucleoid protein H-NS.Yoshida T., Yamashino T., Ueguchi C., Mizuno T.Biosci. Biotechnol. Biochem. 57:1568-1569(1993) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.Link A.J., Robison K., Church G.M.Electrophoresis 18:1259-1313(1997) Escherichia coli proteome analysis using the gene-protein database.VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.Electrophoresis 18:1243-1251(1997) The response to stationary-phase stress conditions in Escherichia coli role and regulation of the glutamic acid decarboxylase system.De Biase D., Tramonti A., Bossa F., Visca P.Mol. Microbiol. 32:1198-1211(1999) Functional characterization and regulation of gadX, a gene encoding an AraC/XylS-like transcriptional activator of the Escherichia coli glutamic acid decarboxylase system.Tramonti A., Visca P., De Canio M., Falconi M., De Biase D.J. Bacteriol. 184:2603-2613(2002) Escherichia coli gene expression responsive to levels of the response regulator EvgA.Masuda N., Church G.M.J. Bacteriol. 184:6225-6234(2002) Collaborative regulation of Escherichia coli glutamate-dependent acid resistance by two AraC-like regulators, GadX and GadW (YhiW) .Ma Z., Richard H., Tucker D.L., Conway T., Foster J.W.J. Bacteriol. 184:7001-7012(2002) Transcriptional expression of Escherichia coli glutamate-dependent acid resistance genes gadA and gadBC in an hns rpoS mutant.Waterman S.R., Small P.L.C.J. Bacteriol. 185:4644-4647(2003) Regulatory network of acid resistance genes in Escherichia coli.Masuda N., Church G.M.Mol. Microbiol. 48:699-712(2003) GadE (YhiE) activates glutamate decarboxylase-dependent acid resistance in Escherichia coli K-12.Ma Z., Gong S., Richard H., Tucker D.L., Conway T., Foster J.W.Mol. Microbiol. 49:1309-1320(2003) Structure of the complex of Escherichia coli glutamate decarboxylase (gadA) with glutarate at 2.05 A resolution.Dutyshev D.I., Darii E.L., Fomenkova N.P., Pechik I.V., Polyakov K.M., Nikonov S.V., Andreeva N.S., Sukhareva B.S.Submitted (SEP-2004) to the PDB data bank

NCBI and Uniprot Product Information

• NCBI GI #: 16131389
• NCBI GeneID: 948027
• NCBI Accession #: NP_417974.1
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P69908; P80063; Q2M7H9
• Molecular Weight: 56.7 kDa
• NCBI Official Full Name: glutamate decarboxylase A, PLP-dependent
• NCBI Official Symbol: gadA
• NCBI Official Synonym Symbols: ECK3502; gadS; JW3485
• NCBI Protein Information: glutamate decarboxylase A, PLP-dependent
• UniProt Protein Name: Glutamate decarboxylase alpha
• Protein Family: Glutamate decarboxylase
• UniProt Gene Name: gadA
• UniProt Synonym Gene Names: gadS; GAD-alpha
• UniProt Entry Name: DCEA_ECOLI

NCBI Description

RpoS regulon. [More information is available at EcoGene: EG50009]. GadA, a glutamate decarboxylase enzyme, is part of the glutamate-dependent acid resistance system 2 (AR2) which confers resistance to extreme acid conditions. [More information is available at EcoCyc: EG50009].

Uniprot Description

Converts glutamate to gamma-aminobutyrate (GABA), consuming one intracellular proton in the reaction. The gad system helps to maintain a near-neutral intracellular pH when cells are exposed to extremely acidic conditions. The ability to survive transit through the acidic conditions of the stomach is essential for successful colonization of the mammalian host by commensal and pathogenic bacteria.