ARF3 recombinant protein

ARF3 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121790

• Purity: >85% as determined by SDS-PAGE
• Synonyms: ARF3; ARF3 Protein; Human; Recombinant (His Tag); Human ARF3/ADP-ribosylation factor 3 Protein (His Tag); ADP-ribosylation factor 3; ARF3 recombinant protein

• Host: E Coli
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 10% glycerol, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Lys181

• Species: Human
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human ARF3 (P61204)(Met1-Lys181) was expressed with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for ARF3 recombinant protein

Background: ARF3, also known as ADP-ribosylation factor 3, belongs to the RAS superfamily. Members of this family include ARF1, ARF2, ARF3, ARF4, ARF5 and ARF6. ARF3 gene is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. ARF3 functions as an allosteric activator of the cholera toxin subunit, an ADP-ribosyltransferase. It is involved in protein trafficking and may modulate vesicle budding and uncoating within the Golgi apparatus.

References

Hirai M. et al., 1997, Genomics. 34 (2): 263-5. Kanoh H. et al., 1997, J Biol Chem. 272 (9): 5421-9. Boman. et al., 2002, Mol Biol Cell. 13 (9): 3078-95.

NCBI and Uniprot Product Information

• NCBI GI #: 4502203
• NCBI GeneID: 377
• NCBI Accession #: NP_001650.1
• NCBI GenBank Nucleotide #: NM_001659.2
• UniProt Accession #: P61204; P16587; A8K6G8; B7ZB63
• Molecular Weight: 16,145 Da
• NCBI Official Full Name: ADP-ribosylation factor 3
• NCBI Official Synonym Full Names: ADP-ribosylation factor 3
• NCBI Official Symbol: ARF3
• NCBI Protein Information: ADP-ribosylation factor 3; small GTP binding protein
• UniProt Protein Name: ADP-ribosylation factor 3
• Protein Family: Auxin response factor
• UniProt Gene Name: ARF3
• UniProt Entry Name: ARF3_HUMAN

NCBI Description

ADP-ribosylation factor 3 (ARF3) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6) and members of each class share a common gene organization. The ARF3 gene contains five exons and four introns. [provided by RefSeq, Jul 2008]

Uniprot Description

ARF3: GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Belongs to the small GTPase superfamily. Arf family.

Protein type: G protein; G protein, monomeric; G protein, monomeric, ARF; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: Golgi membrane; perinuclear region of cytoplasm

Molecular Function: GTPase activity; GTP binding

Biological Process: vesicle-mediated transport; protein transport; small GTPase mediated signal transduction; phospholipid metabolic process; phosphatidylinositol biosynthetic process