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Testing Data

Rabbit ARF3 Polyclonal Antibody | anti-ARF3 antibody

Anti-ARF3 Antibody

Reactivity
Human. Predicted: Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
ARF3; Polyclonal Antibody; Anti-ARF3 Antibody; anti-ARF3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human. Predicted: Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Affinity purified
Form/Format
Liquid
Concentration
100ug/100ul (varies by lot)
Sequence Length
3518
Applicable Applications for anti-ARF3 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
ELISA titer: 1:20,000-1:80,000
Western blot analysis: 1:1,000-1:2,000
Immunohistochemistry: 1:100-1:500
Optimal dilutions/concentrations should be determined by the end user.
Storage Buffer
PBS, pH 7.4 with 0.02% Sodium Azide
Immunogen
Rabbit polyclonal ARF3 (1) antibody was raised against a recombinated human ARF3 protein 2-181aa (BC007647).
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Testing Data
Related Product Information for anti-ARF3 antibody
ADP-ribosylation factor 3 (ARF3) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6) and members of each class share a common gene organization. The ARF3 gene contains five exons and four introns. [provided by RefSeq, Jul 2008]
References
(1) Bailey,S.D., Xie,C., Do,R., et al. Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study. Diabetes Care 33 (10), 2250-2253 (2010).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
377
Molecular Weight
16,145 Da
NCBI Official Full Name
Homo sapiens ADP-ribosylation factor 3, mRNA
NCBI Official Synonym Full Names
ADP-ribosylation factor 3
NCBI Official Symbol
ARF3
NCBI Protein Information
ADP-ribosylation factor 3; small GTP binding protein
Protein Family
UniProt Gene Name
ARF3
UniProt Entry Name
ARF3_HUMAN

NCBI Description

ADP-ribosylation factor 3 (ARF3) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6) and members of each class share a common gene organization. The ARF3 gene contains five exons and four introns. [provided by RefSeq, Jul 2008]

Uniprot Description

ARF3: GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Belongs to the small GTPase superfamily. Arf family.

Protein type: G protein; Motility/polarity/chemotaxis; G protein, monomeric; G protein, monomeric, ARF

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: Golgi membrane; perinuclear region of cytoplasm

Molecular Function: GTPase activity; GTP binding

Biological Process: vesicle-mediated transport; protein transport; phospholipid metabolic process; small GTPase mediated signal transduction; phosphatidylinositol biosynthetic process

Research Articles on ARF3

Similar Products

Product Notes

The ARF3 arf3 (Catalog #AAA416805) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ARF3 Antibody reacts with Human. Predicted: Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ARF3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). ELISA titer: 1:20,000-1:80,000 Western blot analysis: 1:1,000-1:2,000 Immunohistochemistry: 1:100-1:500 Optimal dilutions/concentrations should be determined by the end user. Researchers should empirically determine the suitability of the ARF3 arf3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARF3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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